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Publications associées (32)

Engineering synthetic adhesins for biophysical investigation and rewiring of host-microbe interactions

Host attachment is often a critical step in the onset of pathogenesis. To attach to host cells, bacteria have evolved a range of adhesins that bind to specific receptors. Some of these adhesins have been thoroughly characterized using biochemical technique ...

EPFL2022

Akt Is S-Palmitoylated: A New Layer of Regulation for Akt

Françoise Gisou van der Goot Grunberg, Laurence Gouzi Abrami

The protein kinase Akt/PKB participates in a great variety of processes, including translation, cell proliferation and survival, as well as malignant transformation and viral infection. In the last few years, novel Akt posttranslational modifications have ...

FRONTIERS MEDIA SA2021

A genome-scale metabolic model of Saccharomyces cerevisiae that integrates expression constraints and reaction thermodynamics

Vassily Hatzimanikatis, Ljubisa Miskovic, Maria Masid Barcon, Mohammadomid Oftadeh, Pierre Guy Rémy Salvy

Eukaryotic organisms play an important role in industrial biotechnology, from the production of fuels and commodity chemicals to therapeutic proteins. To optimize these industrial systems, a mathematical approach can be used to integrate the description of ...

NATURE PORTFOLIO2021

Absolute quantification of pceABCT gene products and characterization of the membrane protein complex responsible for the reduction of tetrachloroethene in Dehalobacter restrictus

Christof Holliger, Julien Maillard, Adrian Schmid, Lorenzo Cimmino

The pceABCT gene cluster from Dehalobacter restrictus is one model system for tetrachloroethene (PCE) respiration. In the present study we aim to decipher the stoichiometry of the pceABCT gene products and the composition of PceA-containing membrane-bound ...

2021

Palmitoylated acyl protein thioesterase APT2 deforms membranes to extract substrate acyl chains

Françoise Gisou van der Goot Grunberg, Matteo Dal Peraro, Laurence Gouzi Abrami, Florence Pojer, Sylvia Ho, Francisco De Magalhães Sarmento R De Mesquita, Muhammad Umair Anwar, Patrick Alain Sandoz, Martina Audagnotto, Giulia Fonti

Acyl protein thioesterase APT2 interacts with membranes via its charged beta-tongue, becomes palmitoylated by ZDHHC3/7 and deforms the bilayer to extract substrate acyl chains. APT2 deacylation leads to its membrane release and degradation. Many biochemica ...

NATURE RESEARCH2021

Disruption of mitochondrial redox homeostasis by enzymatic activation of a trialkylphosphine probe

Pablo Rivera Fuentes, Jade Noémie Nam Nguyen

Redox homeostasis is essential for cell function and its disruption is associated with multiple pathologies. Redox balance is largely regulated by the relative concentrations of reduced and oxidized glutathione. In eukaryotic cells, this ratio is different ...

ROYAL SOC CHEMISTRY2021

Nanomechanical bio-sensing for fast and reliable detection of viability and susceptibility of microorganisms

Giovanni Dietler, Sandor Kasas, María Inés Villalba, Leonardo Venturelli, Raphaël Foschia

The metabolic activity of living organisms results in cellular motion on a nanometric scale that can be efficiently detected by micro- and nano- fabricated sensors. Quartz crystal microbalance and atomic force microscopy (AFM) inspired techniques have rece ...

2021

Analysis and Modeling of the Dynamics of Traction Forces during Cell Polarization and their Role in the Organization of Edge Activity

Zeno Messi

The capacity to break symmetry and organize activity to move directionally is a fundamental property of eukaryotic cells. To explain the organization of cell-edge activity, models commonly rely on front-to-back gradients of functional components or regulat ...

EPFL2021

Microfluidics and cell-free synthetic biology in the development of new diagnostic and therapeutic platforms

Grégoire Michielin

In phenylketonuria, absence or malfunction of the phenylalanine hydroxylase enzyme results in toxic accumulation of phenylalanine in the body. An injectable recombinant enzyme therapy was recently approved and has the potential to improve the quality of li ...

EPFL2021

Organisation and dynamics of mitochondria and their RNA.

Timo Henry René Rey

The organisation of molecules into dynamic cells, and collaboration of many of those cells over a billion years led to the evolution of human life. During the last century, biologists then began to unravel the marvels of cellular organisation with ever in ...

EPFL2021

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