Concept

Réaction en chaîne par polymérase

Publications associées (233)

Hardware-software design of a smart sensor for fully-electronic DNA hybridization detection

This paper describes the design of a smart sensor for label-free detection of DNA hybridization. The sensor is based on a direct electrical transduction principle: it measures impedance variation at the interface between a bio-functionalized electrode and ...

2005

Exploring the diversity of metabolism

Vassily Hatzimanikatis

We describe the development of a computational approach for the identification of every possible biochem. reaction from a given set of enzyme reaction rules that allows the de novo synthesis of metabolic pathways composed of these reactions, and the evalua ...

2005

Human protection of telomeres 1 (POT1) is a negative regulator of telomerase activity in vitro

Joachim Lingner

The telomeric single-strand DNA binding protein protection of telomeres 1 (POT1) protects telomeres from rapid degradation in Schizosaccharomyces pombe and has been implicated in positive and negative telomere length regulation in humans. Human POT1 appear ...

2005

Synergy between interstitial flow and VEGF directs capillary morphogenesis in vitro through a gradient amplification mechanism

Melody Swartz, Federica Boschetti, Mark Fleury

Cell organization is largely orchestrated by extracellular gradients of morphogenetic proteins. VEGF, an essential factor for capillary formation, is stored in the extracellular matrix, but the mechanisms by which it and other matrix-bound morphogens are m ...

2005

Neuropeptide and calcium-binding protein gene expression profiles predict neuronal anatomical type in the juvenile rat

Henry Markram

Neocortical neurones can be classified according to several independent criteria: morphological, physiological, and molecular expression (neuropeptides (NPs) and/or calcium-binding proteins (CaBPs)). While it has been suggested that particular NPs and CaBP ...

2005

Exploring the diversity of complex metabolic networks

Vassily Hatzimanikatis

Motivation: Metab., the network of chem. reactions that make life possible, is one of the most complex processes in nature. We describe here the development of a computational approach for the identification of every possible biochem. reaction from a given ...

2005

Development of a real-time PCR method for quantification of the three genera Dehalobacter, Dehalococcoides, and Desulfitobacterium in microbial communities

Christof Holliger, Julien Maillard, Katia Szynalski

We developed standard curves based on plasmids containing a 16S rRNA gene of a member of one of the three genera Dehalobacter, Desulfitobacterium, and Dehalococcoides. A large difference in amplification efficiency between the standard curves was observed ...

2004

Development of degenerate and specific PCR primers for the detection and isolation of known and putative chloroethene reductive dehalogenase genes

Christof Holliger, Julien Maillard

Degenerate and specific PCR primers were designed for the detection of chloroethene reductive dehalogenases (CE-RDase), the key enzymes of chloroethene dehalorespiration, based on sequence information of three CE-RDases and three chlorophenol (CP) RDases. ...

2004

Quantitative rt-PCR analysis of uncoupling protein isoforms in mouse brain cortex: methodological optimization and comparison of expression with brown adipose tissue and skeletal muscle

Pierre Magistretti, Sylvain Eric Lengacher, Luc Pellerin

Uncoupling proteins (UCPs) present in the inner mitochondrial membrane are involved in uncoupling respiration from ATP synthesis. Five UCP isoforms have been identified but information about their presence and level of expression in the central nervous sys ...

2004

Exploring the Capabilities of Nucleic Acid Polymerases by Use of Directed Evolution

Kai Johnsson, Petra Tafelmeyer

A review. Directed evolution consists of repetitive cycles of random mutagenesis of the protein/peptide sequence followed by screening or selection of candidates with desired properties. Many different approaches are used to introduce mutations into a gene ...

Wiley2004

Graph Chatbot

Chattez avec Graph Search

Posez n’importe quelle question sur les cours, conférences, exercices, recherches, actualités, etc. de l’EPFL ou essayez les exemples de questions ci-dessous.

AVERTISSEMENT : Le chatbot Graph n'est pas programmé pour fournir des réponses explicites ou catégoriques à vos questions. Il transforme plutôt vos questions en demandes API qui sont distribuées aux différents services informatiques officiellement administrés par l'EPFL. Son but est uniquement de collecter et de recommander des références pertinentes à des contenus que vous pouvez explorer pour vous aider à répondre à vos questions.

Connectez-vous pour utiliser Chat avec Graph Search