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Calcium phosphate transfection is a widely used method to produce recombinant proteins in mammalian cells. However, the mechanisms involved in plasmid DNA transfer to the nucleus of the transfected cells remain poorly understood and result in great variati ...
Genetic intervention in cell-cycle regulation is a promising strategy to obtain mammalian cell culture proliferation in the absence of exogenous growth factors. In order to gain insights into this approach, known interactions among the four proteins cyclin ...
There are currently two methods for maintaining cultured mammalian cells, continuous passage at 37 degrees C and freezing in small batches. We investigated a third approach, the "pausing" of cells for days or weeks at temperatures below 37 degrees C in a v ...
A new scalable reactor was developed by applying a novel mixing principle that allows the large-scale cultivation of mammalian cells simply with surface aeration using air owing to increased liquid–gas transfer compared to standard stirred-tank bioreactors ...
