Concept

Genetically modified brinjal

The genetically modified brinjal is a suite of transgenic brinjals (also known as an eggplant or aubergine) created by inserting a crystal protein gene (Cry1Ac) from the soil bacterium Bacillus thuringiensis into the genome of various brinjal cultivars. The insertion of the gene, along with other genetic elements such as promoters, terminators and an antibiotic resistance marker gene into the brinjal plant is accomplished using Agrobacterium-mediated genetic transformation. The Bt brinjal has been developed to give resistance against lepidopteron insects, in particular the Brinjal Fruit and Shoot Borer (Leucinodes orbonalis)(FSB) by forming pores in the digestive system. Mahyco, an Indian seed company based in Jalna, Maharashtra, has developed the Bt brinjal. The genetically modified brinjal event is termed Event EE 1, and Mahyco have also applied for approval of two brinjal hybrids. The Event EE 1 was introgressed by plant breeding into various local varieties by University of Agricultural Sciences, Dharwad and Tamil Nadu Agricultural University, Coimbatore. Some of the cultivars of brinjal include: Malpur local, Manjari gota, Kudachi local, Udupi local, 112 GO, and Pabkavi local. It was approved for commercialization in India in 2009, but - after an apparent public outcry and rounds of debates in which representatives from Mahyco, the scientific community, and NGO's spoke on the topic - the then Indian Environment Minister, Jairam Ramesh, facilitated a moratorium on its release until further, unspecified, tests were conducted. Bt brinjal was approved for commercial release in Bangladesh in 2013. By 2021 it was cultivated by nearly 65,000 farmers with nearly 6x increase in net returns, with around 20% of farmers using seeds from previous seasons. Mahyco licensed and used the cry1Ac gene obtained from Monsanto and two supporting genes (nptII and aad). The cry1Ac gene is under the transcriptional control of an enhanced cauliflower mosaic virus 35S (CaMV35S) promoter, which ensures the gene is expressed in all the brinjal's tissue throughout its complete life cycle.

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