Concept

Agarose

Publications associées (36)

Capillary electrophoresis immunoassay using magnetic beads

Hubert Girault, Anne-Laure Gassner, Hong-Xu Chen

Protein A-coated magnetic beads (0.3 μm) have been trapped in a small portion of a neutrally coated capillary (50 μm id). Anti-β-lactoglobulin (β-LG) antibodies have then been immobilized on the beads through strong affinity with protein A to subsequently ...

2008

The role of cisplatin and NAMI-A plasma-protein interactions in relation to combination therapy

Paul Joseph Dyson, Gianni Sava

The aim of the study is to evaluate the differences of protein binding of NAMI-A, a new ruthenium drug endowed with selective antimetastatic properties, and of cisplatin and to ascertain the possibility to use two drugs based on heavy metals in combination ...

2006

The use of RNA interference to increase PEI-mediated transient gene expression in mammalian cells

Martin Bertschinger

DNA uptake by polyethylenimine (PEI)-mediated transfection was investigated in Chinese hamster ovary (CHO DG44) cells. Rapid DNA uptake was observed with the maximum occurring during the first 45-60 min after addition of PEI-DNA complexes to cells. With lo ...

EPFL2006

Hexachlorocyclohexane-degrading bacterial strains Sphingomonas paucimobilis B90A, UT26 and Sp+, having similar lin genes, represent three distinct species, Sphingobium indicum sp. nov., Sphingobium japonicum sp. nov. and Sphingobium francense sp. nov., and reclassification of [Sphingomonas] chungbukensis as Sphingobium chungbukense comb. nov.

Christof Holliger, Mithlesh Kumar

Three strains of Sphingomonas paucimobilis, B90A, UT26 and Sp+, isolated from different geographical locations, were found to degrade hexachlorocyclohexane. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these strains do not fall in ...

Society for General Microbiology2005

Towards a fully synthetic substitute of alginate: Optimization of a thermal gelation/chemical cross-linking scheme ("tandem" gelation) for the production of beads and liquid-core capsules

Jeffrey Alan Hubbell, Nicola Tirelli

Fully synthetic polymers were used for the prepn. of hydrogel beads and capsules, in a processing scheme that, originally designed for calcium alginate, was adapted to a "tandem" process, that is the combination a phys. gelation with a chem. crosslinking ...

2004

Intracellular targets of cyclin-dependent kinase inhibitors: identification by affinity chromatography using immobilised inhibitors

BACKGROUND: Chemical inhibitors of cyclin-dependent kinases (CDKs) have great therapeutic potential against various proliferative and neurodegenerative disorders. Olomoucine, a 2,6,9-trisubstituted purine, has been optimized for activity against CDK1/cycli ...

Elsevier2000

Three-dimensional extracellular matrix engineering in the nervous system

Patrick Aebischer

Growing neurites are guided through their environment during development and regeneration via different cellular and extracellular matrix (ECM) molecular cues. To mimic cell-matrix interactions, a three-dimensional (3D) hydrogel-based ECM equivalent contai ...

1998

Genome mapping of Clostridium perfringens strains with I-CeuI shows many virulence genes to be plasmid-borne

Stewart Cole

The intron-encoded endonuclease I-CeuI from Chlamydomonas eugametos was shown to cleave the circular chromosomes of all Clostridium perfringens strains examined at single sites in the rRNA operons, thereby generating ten fragments suitable for the rapid ma ...

1996

Design and characterization of a phantom that simultaneously simulates tissue optical properties between 400 and 650 nm

Hubert van den Bergh, Georges Wagnières, Matthieu Philippe Emmanuel Zellweger

The design and characterization of optical phantoms which have the same absorption and scattering characteristics as biological tissues in a broad spectral window (between 400 and 650 nm) are presented. These low cost phantoms use agarose dissolved in wate ...

1996

Hydrogel-based three-dimensional matrix for neural cells

Patrick Aebischer, Nicolas Bouche

The ability to organize cells in three dimensions (3D) is an important component of tissue engineering. This study sought to develop an extracellular matrix (ECM) equivalent with a physicochemical structure capable of supporting neurite extension from prim ...

1995

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