Colonisation de l'espace | EPFL Graph Search

thumb|upright=1.2|Habitat spatial en construction de type tore de Stanford, dessin d'artiste pour la NASA, 1975.|alt=Vue d'artiste d'un habitat spatial. La colonisation de l'espace, ou colonisation spatiale, est au-delà d'un sujet classique de fiction, un projet astronautique d'habitation humaine permanente et en grande partie auto-suffisante en dehors de la Terre. Elle est liée à l'exploration spatiale. Plusieurs groupes de développement de la NASA, de l'ESA, des Agences spatiales russe et chinoise ainsi que d'autres scientifiques ont étudié la faisabilité de projets de colonies spatiales en divers endroits du Système solaire. Bien qu'ils aient déterminé qu'il y a des matières premières exploitables sur la Lune et les astéroïdes géocroiseurs, que l'énergie solaire est disponible en grande quantité et qu'aucune nouvelle découverte scientifique majeure n'est nécessaire, ils ont évalué qu'il faudrait des prouesses techniques d'ingénierie, une meilleure connaissance de l'adaptation huma

Nutrient Availability Does Not Affect Community Assembly in Root-Associated Fungi but Determines Fungal Effects on Plant Growth

Bing Bai, Florian Frédéric Vincent Breider

Nonmycorrhizal root-colonizing fungi are key determinants of plant growth, driving processes ranging from pathogenesis to stress alleviation. Evidence suggests that they might also facilitate host access to soil nutrients in a mycorrhiza-like manner, but the extent of their direct contribution to plant nutrition is unknown. To study how widespread such capacity is across root-colonizing fungi, we surveyed soils in nutrient-limiting habitats using plant baits to look for fungal community changes in response to nutrient conditions. We established a fungal culture collection and used Arabidopsis thaliana inoculation bioassays to assess the ability of fungi to facilitate host’s growth in the presence of organic nutrients unavailable to plants. Plant baits captured a representation of fungal communities extant in natural habitats and showed that nutrient limitation has little influence on community assembly. Arabidopsis thaliana inoculated with 31 phylogenetically diverse fungi exhibited a consistent fungus-driven growth promotion when supplied with organic nutrients compared to untreated plants. However, direct phosphorus measurement and RNA-seq data did not support enhanced nutrient uptake but rather that growth effects may result from changes in the plant’s immune response to colonization. The widespread and consistent host responses to fungal colonization suggest that distinct, locally adapted nonmycorrhizal fungi affect plant performance across habitats.

2022

A Mini-Atlas of diatom frustule electron microscopy images at different magnifications

Joris Pierre Everaerts, Chrysanthi Papadaki

Diatom algae are active and efficient photosynthesizing single cell organisms responsible for a quarter of biomass and a quarter of oxygen release on the Earth surface. Diatoms form an enormously diverse class of microorganisms possessing stiff and strong, durable exoskeletons made from hydrated amorphous silica forming neat 3D nanostructures, and displaying hierarchical organization up to the scale of tens of micrometres. In our ongoing research into the structure and properties of diatoms we observed their colonization patterns on various surfaces, including polymers (PE, PP, PC and PETF) and silicon. This process can be guided by purposeful surface patterning to introduce pits, grooves, and ledges. Guided colonization opens the prospect of assembly and harvesting diatom frustules for applications in micro- and nanoelectro-mechanical systems (MEMS and NEMS). Additionally, the wide range and specificity of diatoms opens the possibility of using them as tags and markers that are below the level of visibility by naked eye, but present specific spectroscopic fingerprints in visible light and UV ranges. Diatom `tags' can also be read using high magnification imaging using Scanning Electron Microscopy (SEM). As a contributory guidance to morphological diversity of diatoms we present a mini-atlas of diatom in the form of high resolution SEM images. (C) 2020 Elsevier Ltd. All rights reserved.

ELSEVIER2020

Functional intestinal bile acid 7-alpha-dehydroxylation by Clostridium scindens associated with protection from C. difficile infection in a gnotobiotic mouse model

Rizlan Bernier-Latmani, Lyne Desharnais, Laure Menin

Bile acids, important mediators of lipid absorption, also act as hormone-like regulators and as antimicrobial molecules. In all these functions their potency is modulated by a variety of chemical modifications catalyzed by bacteria of the healthy gut microbiota, generating a complex variety of secondary bile acids. Intestinal commensal organisms are well adapted to normal concentrations of bile acids in the gut. In contrast, physiological concentrations of the various intestinal bile acid species play an important role in the resistance to intestinal colonization by pathogens such as Clostridium difficile. Antibiotic therapy can perturb the gut microbiota and thereby impair the production of protective secondary bile acids. The most important bile acid transformation is 7 alpha-dehydroxylation, producing deoxycholic acid (DCA) and lithocholic acid (LCA). The enzymatic pathway carrying out 7 alpha-dehydroxylation is restricted to a narrow phylogenetic group of commensal bacteria, the best-characterized of which is Clostridium scindens. Like many other intestinal commensal species, 7 alpha-dehydroxylating bacteria are understudied in vivo. Conventional animals contain variable and uncharacterized indigenous 7 alpha-dehydroxylating organisms that cannot be selectively removed, making controlled colonization with a specific strain in the context of an undisturbed microbiota unfeasible. In the present study, we used a recently established, standardized gnotobiotic mouse model that is stably associated with a simplified murine 12-species "oligo-mouse microbiota" (Oligo-MM12). It is representative of the major murine intestinal bacterial phyla, but is deficient for 7 alpha-dehydroxylation. We find that the Oligo-MM12 consortium carries out bile acid deconjugation, a prerequisite for 7 alpha-dehydroxylation, and confers no resistance to C. difficile infection (CDI). Amendment of Oligo-MM12 with C. scindens normalized the large intestinal bile acid composition by reconstituting Ty-dehydroxylation. These changes had only minor effects on the composition of the native Oligo-MM12, but significantly decreased early large intestinal C. difficile colonization and pathogenesis. The delayed pathogenesis of C. difficile in C. scindens-colonized mice was associated with breakdown of cecal microbial bile acid transformation.

Frontiers Media Sa2016

Functional intestinal bile acid 7-alpha-dehydroxylation by Clostridium scindens associated with protection from C. difficile infection in a gnotobiotic mouse model

Rizlan Bernier-Latmani, Lyne Desharnais, Laure Menin

Frontiers Media Sa2016