Concept

Molecular cloning

Publications associées (174)

Mild hypothermia improves transient gene expression yields several fold in Chinese hamster ovary cells

Florian Maria Wurm, David Hacker, Stéphanie Tissot, Sarah Wulhfard, Sophie Bouchet, Jean Cevey

Large-scale transient gene expression (TGE) in mammalian cells is a rapid method to generate recombinant proteins, but the volumetric productivity for secreted proteins is still more than an order of magnitude lower than the yields typically achieved with ...

2008

Gene delivery from responsive hyaluronic acid hydrogels for directed angiogenesis

Arnaud Felber

Tissue Engineering points to maintain, restore or to improve and create new tissues. This purpose typically involves the selection of a natural or synthetic biomaterial as a scaffold to create the biological substitutes. Furthermore, angiogenesis, the grow ...

2008

Strategies in non-viral gene delivery: "to overcome serum effect"

Yun Suk Jo

Throughout these last years, many synthetic polymers have been developed for non-viral gene delivery systems in attempt to overcome potential shortcomings of viral vectors. However, it is still difficult to achieve highly efficient transfection under physi ...

2008

Methods of nucleic acid amplification and sequencing

Gerardo Turcatti

Methods for amplification and sequencing of at least one nucleic acid comprising the following steps: (1) forming at least one nucleic acid template comprising the nucleic acid(s) to be amplified or sequenced, wherein said nucleic acid(s) to be amplified o ...

2008

One-step refolding and purification of disulfide-containing proteins with a C-terminal MESNA thioester

Maartje Martina Cornelia Bastings

Background Expression systems based on self-cleavable intein domains allow the generation of recombinant proteins with a C-terminal thioester. This uniquely reactive C-terminus can be used in native chemical ligation reactions to introduce synthetic groups ...

BioMed Central2008

An affinity oligonucleotide displacement strategy to purify ribonucleoprotein complexes applied to human telomerase

Joachim Lingner

Antisense oligonucleotides have been used to study the structure and function of small nuclear ribonucleoprotein (snRNP) complexes and were adapted and modified for the purification of a variety of RNPs. We describe methods for recombinant expression and r ...

Humana Press2008

The extracellular nuclease Dns and its role in natural transformation of Vibrio cholerae

Melanie Blokesch

Free extracellular DNA is abundant in many aquatic environments. While much of this DNA will be degraded by nucleases secreted by the surrounding microbial community, some is available as transforming material that can be taken up by naturally competent ba ...

American Society for Microbiology2008

Coexpression of acidic fibroblast growth factor enhances specific productivity and antibody titers in transiently transfected HEK293 cells

Florian Maria Wurm, Gaurav Backliwal, Sébastien Chenuet

Recombinant proteins are of great commercial and scientific interest. However, most current production methods using mammalian cells involve the time- and labor-intensive step of creating stable cell lines. Although production methods based on transient ge ...

2008

Probabilistic base calling of Solexa sequencing data

Felix Naef, Jacques Rougemont, Christian Iseli, Arnaud Amzallag, Ioannis Xenarios

BACKGROUND: Solexa/Illumina short-read ultra-high throughput DNA sequencing technology produces millions of short tags (up to 36 bases) by parallel sequencing-by-synthesis of DNA colonies. The processing and statistical analysis of such high-throughput dat ...

BioMed Central2008

Bio-inspired self-organizing cellular systems

Daniel Mange, Joël Rossier, Fabien Vannel

Bio-inspiration borrows three properties characteristic of living organisms: multicellular architecture, cellular division, and cellular differentiation. Implemented in silicon according to these properties, our self-organizing systems are able to grow, to ...

2008

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