Concept

3'-UTR

Publications associées (83)

Alterations of the 5 ' Untranslated Region of SLC16A12 Lead to Age-Related Cataract

PURPOSE. Knowledge of genetic factors predisposing to age-related cataract is very limited. The aim of this study was to identify DNA sequences that either lead to or predispose for this disease. ...

2010

KAP1 controls endogenous retroviruses in embryonic stem cells

Didier Trono, Daniel Constam, Jacques Rougemont, Sonia Verp, Séverine Reynard, Helen Mary Rowe, Johan Jakobsson, Pierre Maillard, Hillary Layard-Liesching

More than forty per cent of the mammalian genome is derived from retroelements, of which about one-quarter are endogenous retroviruses (ERVs). Some are still active, notably in mice the highly polymorphic early transposon (ETn)/MusD and intracisternal A-ty ...

Nature Publishing Group2010

Conditional gene targeting of the ENaC subunit genes Scnn1b and Scnn1g

Friedrich Beermann, Roch-Philippe Charles

Epithelial sodium channels (ENaC) are members of the degenerin/ENaC superfamily of non-voltage-gated, highly amiloride-sensitive cation channels that are composed of three subunits (alpha-, beta- and gamma- ENaC). Since complete gene inactivation of the be ...

2009

Transcriptional and post-transcriptional regulation of endothelin-1 gene by shear stress

Nikolaos Stergiopoulos, Rafaela Fernandes Da Silva, Paolo Silacci

Objective Plaque-prone areas are exposed to a particular hemodynamic environment characterized by a low mean shear stress value and a cyclic reversal flow. This mechanical environment, also termed oscillatory shear stress (OSS), induces the expression of s ...

2008

Mild hypothermia improves transient gene expression yields several fold in Chinese hamster ovary cells

Florian Maria Wurm, David Hacker, Stéphanie Tissot, Sarah Wulhfard, Sophie Bouchet, Jean Cevey

Large-scale transient gene expression (TGE) in mammalian cells is a rapid method to generate recombinant proteins, but the volumetric productivity for secreted proteins is still more than an order of magnitude lower than the yields typically achieved with ...

2008

Genotypic features of lentivirus transgenic mice

Didier Trono, Denis Duboule, Felix Naef, Frédéric Schütz, Marc-Olivier Sauvain

Lentivector-mediated transgenesis is increasingly used, whether for basic studies as an alternative to pronuclear injection of naked DNA or to test candidate gene therapy vectors. In an effort to characterize the genetic features of this approach, we first ...

American Society for Microbiology2008

Differential translational regulation of IRE-containing mRNAs in Drosophila melanogaster by endogenous IRP and a constitutive human IRP1 mutant

Lukas Kühn

Insects, like vertebrates, express iron regulatory proteins (IRPs) that may regulate proteins in cellular iron storage and energy metabolism. Two mRNAs, an unspliced form of ferritin H mRNA and succinate dehydrogenase subunit b (SDHb) mRNA, are known to co ...

2008

Molecular mechanism of hepcidin deficiency in a patient with juvenile hemochromatosis

Didier Trono, Bastien Mangeat

We describe a point mutation creating an additional ATG codon in the 5' untranslated region (UTR) of the HAMP gene, in a patient with juvenile hemochromatosis. By transient in vitro transfection studies, we provide evidence that the additional ATG is funct ...

Pensiero Scientifico / Ferrata Storti Foundation2007

Destabilization of interleukin-6 mRNA requires a putative RNA stem-loop structure, an AU-rich element, and the RNA-binding protein AUF1

Lukas Kühn, Barbara Grisoni-Neupert

Interleukin-6 mRNA is unstable and degraded with a half-life of 30 min. Instability determinants can entirely be attributed to the 3' untranslated region. By grafting segments of this region to stable green fluorescent protein mRNA and subsequent scanning ...

2006

Similarities and differences of polyadenylation signals in human and fly

Felix Naef, Philipp Bucher

ABSTRACT: BACKGROUND: Cleavage of messenger RNA (mRNA) precursors is an essential step in mRNA maturation. The signal recognized by the cleavage enzyme complex has been characterized as an A rich region upstream of the cleavage site containing a motif with ...

2006

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