Concept

Genetic engineering techniques

Publications associées (81)

Spatio-temporal synchronization of biological oscillators

The circadian clock is a cell-autonomous and self-sustained oscillator with a period of about 24 hours that controls many aspects of cellular physiology. The cell cycle is also a fundamental periodic process, with a period in the range of one day in mammal ...

EPFL2014

Overexpression of the tcp Gene Cluster Using the T7 RNA Polymerase/Promoter System and Natural Transformation-Mediated Genetic Engineering of Vibrio cholerae

Melanie Blokesch

The human pathogen and aquatic bacterium Vibrio cholerae belongs to the group of naturally competent bacteria. This developmental program allows the bacterium to take up free DNA from its surrounding followed by a homologous recombination event, which allo ...

Public Library of Science2013

TAL Effectors Specificity Stems from Negative Discrimination

Matteo Dal Peraro, Marco Stenta, Basile Isidore Martin Wicky

Transcription Activator-Like (TAL) effectors are DNA-binding proteins secreted by phytopathogenic bacteria that interfere with native cellular functions by binding to plant DNA promoters. The key element of their architecture is a domain of tandem-repeats ...

Public Library of Science2013

A novel encapsulation device for peripheral implantation of antibody-secreting cells

Aurélien Lathuilière

Alzheimer’s disease (AD) is the most prevalent form of dementia in the elderly. As populations are aging in most parts of the world, the number of people with Alzheimer’s disease is expected to dramatically increase. This devastating neurodegenerative diso ...

EPFL2013

Characterisation of ypa1 and ypa2, the Schizosaccharomyces pombe orthologues of the peptidyl proyl isomerases that activate PP2A, reveals a role for Ypa2p in the regulation of cytokinesis

Viesturs Simanis, Anupama Goyal

The Schizosaccharomyces pombe septation initiation network regulates cytokinesis. Cdc7p is the first kinase in the core septation initiation network; we have screened genetically for SIN regulators by isolating cold-sensitive suppressors of cdc7-24. Our sc ...

2012

TransFLP - A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination

Melanie Blokesch

Several methods are available to manipulate bacterial chromosomes(1-3). Most of these protocols rely on the insertion of conditionally replicative plasmids (e.g. harboring pir-dependent or temperature-sensitive replicons(1,2)). These plasmids are integrate ...

2012

Rapid Synthesis of Defined Eukaryotic Promoter Libraries

Sebastian Maerkl, Arun Stephen Rajkumar

Current gene synthesis methods allow the generation of long segments of dsDNA. We show that these techniques can be used to create synthetic regulatory elements and describe a method for the creation of completely defined, synthetic variants of the PHOS pr ...

Amer Chemical Soc2012

Lentiviral Vector Mediated Transgenesis

Didier Trono, Isabelle Barde, Sonia Verp, Sandra Eloise Kjeldsen

The genetic manipulation of rodents through the generation of fully transgenic animals or via the modification of selective cells or organs is a procedure of paramount importance for biomedical research, either to address fundamental questions or to develo ...

2011

ZFN-Site searches genomes for zinc finger nuclease target sites and off-target sites

Giovanna Ambrosini, Christian Iseli, Philipp Bucher

Background: Zinc Finger Nucleases (ZFNs) are man-made restriction enzymes useful for manipulating genomes by cleaving target DNA sequences. ZFNs allow therapeutic gene correction or creation of genetically modified model organisms. ZFN specificity is not a ...

BioMed Central2011

Cyanobacteria and microalgae: a positive prospect for biofuels

Edgard Gnansounou

Biofuel–bioenergy production has generated intensive interest due to increased concern regarding limited petroleum-based fuel supplies and their contribution to atmospheric CO2 levels. Biofuel research is not just a matter of finding the right type of biom ...

Elsevier2011

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