Concept

Cas9

Publications associées (43)

Monitoring the energy of the metal ion-content plasma-assisted deposition and its implication for bacterial inactivation

Cu-polyester (Cu-PES) was sputtered by high power impulse magnetron sputtering (HIPIMS) and by low energy direct current magnetron sputtering (DCMS). The total amount and distribution of the Ar+, Cu+ and Cu2+ ions were determined as well as the bacterial i ...

ELSEVIER SCIENCE BV2019

Functional analysis of the telomeric long non-coding RNA TERRA

Patricia Renck Nunes

Telomeres are nucleoprotein structures present in the end of linear chromosomes. In humans, a core complex of 6 proteins cap and protect the chromosome ends from being recognized as double-strand breaks and eliciting unwanted DNA damage and repair response ...

EPFL2019

Engineered anti-CRISPR proteins for optogenetic control of CRISPR-Cas9

Bruno Emanuel Ferreira De Sousa Correia, Zander Harteveld

Anti-CRISPR proteins are powerful tools for CRISPR-Cas9 regulation; the ability to precisely modulate their activity could facilitate spatiotemporally confined genome perturbations and uncover fundamental aspects of CRISPR biology. We engineered optogeneti ...

NATURE PUBLISHING GROUP2018

Preservation of visual function in an animal model of retinal degeneration using CRISPR/Cas9 gene editing

Diego Ghezzi, Paola Vagni, Tommaso Marchetti

Aims: Inherited Retinal Disorders represent a difficult target for gene therapy. This study exploits the possibility of using CRISPR/Cas9-based gene editing in photoreceptor progenitors to prevent cell death. We performed all the experiments on Rd10 mice, ...

2018

Efficient cleavage of aryl ether C–O linkages by Rh–Ni and Ru–Ni nanoscale catalysts operating in water

Paul Joseph Dyson, Vassily Hatzimanikatis, Duncan Thomas Lindsay Alexander, Antoine Philippe Van Muyden, Ning Yan, Georgios Savoglidis, Sviatlana Siankevich, Safak Bulut

Bimetallic Ru–Ni and Rh–Ni nanocatalysts coated with a phase transfer agent efficiently cleave aryl ether C–O linkages in water in the presence of hydrogen. For dimeric substrates with weaker C–O linkages, i.e. α-O-4 and β-O-4 bonds, low loadings of the pr ...

2018

Gene editing in photoreceptor progenitors prevents visual function loss in a mouse model of retinal degeneration

Diego Ghezzi, Paola Vagni

Purpose:Currently, there is no known cure for Retinitis pigmentosa (RP). Even if some treatments can slow down the progression of the disease, none of them can effectively stop retinal degeneration. We exploited the possibility of an early intervention in ...

2017

Preventing visual function loss in the rd10 mouse model of retinitis pigmentosa using gene editing

Diego Ghezzi, Paola Vagni

Currently, there is no known cure for retinitis pigmentosa (RP). Even if some treatments can slow down the progression of the disease, none of them can effectively stop retinal degeneration. This study exploits the possibility of an early intervention in p ...

2017

Cullin-RING ubiquitin E3 ligase regulation by the COP9 signalosome

Henning Paul-Julius Stahlberg, Simone Cavadini, Radosav Svetozara Pantelic

The cullin-RING ubiquitin E3 ligase (CRL) family comprises over 200 members in humans. The COP9 signalosome complex (CSN) regulates CRLs by removing their ubiquitin-like activator NEDD8. The CUL4A-RBX1-DDB1-DDB2 complex (CRL4A(DDB2)) monitors the genome fo ...

Springer Nature2016

Principles of Systems Biology, No. 11

Vassily Hatzimanikatis, Jasmin Maria Hafner, Noushin Hadadi, Laurent Potvin-Trottier

This month: AI that learns patterns and facts, new protein-RNA and protein-protein relationships, engineering signaling and metabolism, and more variants of Cas9. ...

Cell Press2016

Key Players in I-DmoI Endonuclease Catalysis Revealed from Structure and Dynamics

Homing endonucleases, such as I-DmoI, specifically recognize and cleave long DNA target sequences (∼20 bp) and are potentially powerful tools for genome manipulation. However, inefficient and off-target DNA cleavage seriously limits specific editing in com ...

American Chemical Society2016

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