Concept
Transcription-mediated amplification
Publications associées (21)
Electronic readout of DNA amplification in nanoliter chambers. Strategies towards highly parallel semiconductor-based nucleic acid amplification testing.
Polymerase chain reaction (PCR) has been the most significant driver in the field of nucleic acid testing (NAT) since its invention. Popularized as an abbreviation by the Covid-19 pandemic, PCR-based methods are the gold standard in the field of diagnostic ...
EPFL2023Electrochemical sensors for the detection of SARS-CoV-2 virus
Somanath Jagannath, Neeraj Kumar
Coronavirus (COVID-19), a deadly pandemic has spread worldwide and created many global health issues. Though methods of its detection are being continuously developed for the early detection and monitoring of COVID-19, still there is need for more novel me ...
ELSEVIER SCIENCE SA2022Development and validation of a multiplex real-time qPCR assay using GMP-grade reagents for leprosy diagnosis
Stewart Cole, Charlotte Avanzi, Thyago Leal Calvo
Author summaryLeprosy is a chronic dermato-neurological disease caused by Mycobacterium leprae, an obligate intracellular bacterium. Diagnosis of leprosy often relies on skin examinations for clinical signs, bacilli staining from skin smears and invasive s ...
PUBLIC LIBRARY SCIENCE2022Isothermal multiple displacement amplification of DNA templates in minimally buffered conditions using phi29 polymerase
Carlotta Guiducci, Enrico Tenaglia
The isothermal amplification of DNA in minimally buffered conditions allows to perform and monitor nucleic acid amplification with minimal technological and operative requirements. We show in this work how phi29 can operate multiple displacement amplificat ...
2018Multiplexed nanoplasmonic biosensor for one-step simultaneous detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine
Hatice Altug, Andrea Cavallini, Alexander Belushkin, Maria Soler Aznar
Development of rapid and multiplexed diagnostic tools is a top priority to address the current epidemic problem of sexually transmitted diseases. Here we introduce a novel nanoplasmonic biosensor for simultaneous detection of the two most common bacterial ...
2017Quantitative Estimation of Biological Cell Surface Receptors by Segmenting Conventional Fluorescence Microscopy Images
Giovanni De Micheli, Sandro Carrara, Danilo Demarchi, Paolo Silacci, Julien Michel Ghaye, Chiara Succa, Guy Vergères
State-of-the-art techniques for measuring and monitoring gene level expression rely on messenger RNA (mRNA) extraction and quantification, usually based on the concept of reverse transcription polymerase chain reaction. In this paper, we take adva ...
Ieee2014Corrinoid auxotrophy in the obligate organohalide-respiring Dehalobacter restrictus strain PER-K23
Christof Holliger, Julien Maillard, Yue Lu, Aamani Rupakula Boyanapalli, Hauke Smidt
Background Corrinoids are an essential cofactor of reductive dehalogenases, the key enzymes of organohalide respiration (OHR). Dehalobacter restrictus is an obligate OHR bacterium (OHRB) able to conserve energy with tetrachloroethene, but is unable to de n ...
2014Corrinoid Auxotrophy in the Obligate Organohalide-Respiring Dehalobacter restrictus strain PER-K23
Christof Holliger, Julien Maillard, Yue Lu, Aamani Rupakula Boyanapalli, Hauke Smidt
Introduction: Corrinoids are an essential cofactor of reductive dehalogenases, the key enzymes of organohalide respiration (OHR). Dehalobacter restrictus strain PER-K23 is an obligate OHR bacterium able to conserve energy with tetrachloroethene, but is una ...
2014Corrinoid auxotrophy in the obligate organohalide respiring Dehalobacter restrictus
Christof Holliger, Julien Maillard, Aamani Rupakula Boyanapalli, Kiyan Shabestary
Background Corrinoids are an essential cofactor of reductive dehalogenases, the key enzymes of the environmental-friendly process of organohalide respiration (OHR). Dehalobacter restrictus strain PER-K23 is an obligate OHR bacterium (OHRB) able to conserve ...
2013Methods of nucleic acid amplification and sequencing
Methods for amplification and sequencing of at least one nucleic acid comprising the following steps: (1) forming at least one nucleic acid template comprising the nucleic acid(s) to be amplified or sequenced, wherein said nucleic acid(s) to be amplified o ...
2008