Concept

Empreinte génétique

Publications associées (219)

A fully electronic label-free DNA sensor chip

This paper presents a microfabricated DNA chip for fully electronic, label-free DNA recognition, based on capacitance measurements. The chip has been fabricated in 0.5-mu m CMOS technology and it features an array of individually addressable sensing sites ...

2007

Interface Layering Phenomena in Capacitance Detection of DNA with Biochips

Giovanni De Micheli, Sandro Carrara, Carlotta Guiducci, Yusuf Leblebici, Luca Benini, Bruno Samori

Reliable DNA detection is of great importance for the development of the Lab-on-chip technology. The effort of the most recent projects on this field is to integrate all necessary operations, such as sample preparation (mixing, PCR amplification) together ...

2007

Controlling DNA capture and propagation through artificial nanopores

Aleksandra Radenovic

Electrophorescing blopolymers across nanopores modulates the ionic current through the pore, revealing the polymer's diameter, length, and conformation. The rapidity of polymer translocation (similar to 30 000 bp/ms) in this geometry greatly limits the inf ...

American Chemical Society2007

Tuning the hydrophobicity of ruthenium(II)-arene (RAPTA) drugs to modify uptake, biomolecular interactions and efficacy

Paul Joseph Dyson, Christian Hartinger, Claudine Scolaro, Gianni Sava

The antitumor activity of the organometallic ruthenium(II)-arene mixed phosphine complexes, [Ru(h6-p-cymene)Cl(PTA)(PPh3)]BF4 1b and [Ru(h6-C6H5CH2CH2OH)Cl(PTA)(PPh3)]BF4 2b (PTA = 1,3,5-triaza-7-phosphaadamantane), have been evaluated in vitro and compare ...

2007

Indoor location for safety application using wireless networks

Phillip Tomé

This paper presents the indoor positioning research activities carried out within the scope of the Liaison project. Most of the work has been performed on WiFi location. WiFi is nowadays widely deployed in buildings such as hotels, hospitals, airports, tra ...

2007

Disassembly of polyethylenimine-DNA particles in vitro: implications for polyethylenimine-mediated DNA delivery

Florian Maria Wurm, David Hacker, Martin Jordan, Martin Bertschinger, Gaurav Backliwal

Here a simple in vitro assay was used to investigate the disassembly of nanoparticles of polyethylenimine (PEI) and DNA. Particles were formed with various PEIs, allowed to mature for 10 min, and then exposed to different competitors (RNA, DNA, BSA or hepa ...

2006

Micro-array device, for DNA recognition, includes micro-sensors to output signals indicating absorption of ultraviolet radiation in micro-location, when traversed by UV beam during hybridization process

Carlotta Guiducci

NOVELTY - The micro-array device has several micro-locations (3) associated with a preset DNA sequence. Several micro-sensors are positioned corresponding to the micro-locations. The micro-sensors output electrical signals indicating the absorption of ultr ...

Univ Alma Mater Studiorum Di Bologna; Ricco B; Guiducci C2006

DNA biosensor using fluorescence microscopy and impedance spectroscopy

Martinus Gijs, Daniel Berdat

Two types of DNA biosensors are presented. Both sensing principles are demonstrated using synthetic oligomer single-stranded DNA (ssDNA) with concentrations in the micromolar range. A first sensor type is based on the detection of fluorescently labeled ssD ...

2006

Multiple glycerol shocks increase the calcium phosphate transfection of non-synchronized CHO cells

Florian Maria Wurm, David Hacker, Martin Bertschinger, Frédéric Grosjean

The exposure of CHO DG44 cells to an osmotic shock, after DNA uptake, results in a cellular volume decrease of approx. 55%. Repetitive osmotic shocks targeted different sub-populations of cells as was demonstrated using two different fluorescent reporter g ...

2006

The use of RNA interference to increase PEI-mediated transient gene expression in mammalian cells

Martin Bertschinger

DNA uptake by polyethylenimine (PEI)-mediated transfection was investigated in Chinese hamster ovary (CHO DG44) cells. Rapid DNA uptake was observed with the maximum occurring during the first 45-60 min after addition of PEI-DNA complexes to cells. With lo ...

EPFL2006

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