Concept

Defocus aberration

Publications associées (59)

Adaptive optics using a liquid crystal spatial light modulator for ultrahigh-resolution optical coherence tomography

Three-dimensional ultrahigh resolution optical coherence tomography (UHR OCT) and adaptive optics (AO) are combined using a liquid crystal programmable phase modulator (PPM) as a correcting device for the first time. AO is required for correcting ocular ab ...

SPIE2006

Fluorescence correlation spectroscopy

Ramachandra Rao

Fluorescence Correlation Spectroscopy (FCS) has become an important tool for measuring diffusion, concentration and molecular interactions in biomolecular systems. Two major requirements for FCS are good collection efficiency and a small and well defined e ...

EPFL2006

Measurement and interpretation of the 3D amplitude point spread function of lenses and microscope objectives

The intensity point spread function (IPSF) is most often used to characterize optical imaging systems. For some imaging applications such as digital holographic microscopy the 3D amplitude point spread function (APSF) more completely describes an optical s ...

EPFL2005

On the Feasibility of Axial Tracking of a Fluorescent Nano-Particle Using a Defocusing Model

Michaël Unser, Dimitri Nestor Alice Van De Ville

The image of a sub-resolution nano-particle in fluorescence microscopy corresponds to a slice of the 3D point spread function (PSF). This slice relates to the out-of-focus distance of the nano-particle. In this paper, we investigate to which extent it is p ...

IEEE2004

Point spread function model for microscopic image deconvolution in digital holographic microscopy

Christian Depeursinge, Etienne Cuche, Mihaela Marian

A simple model is proposed in order to evaluate the complex amplitude point spread function (intensity and phase) of a microscope objective. The model is based on the Fresnel diffraction theory and takes also into account the possible optical aberrations. ...

SPIE2003

Micro-replication of Optical Lenses in Glass using Sol Gel Technology

Martinus Gijs, Abdeljalil Sayah, Virendra Kumar Parashar

A new method to realize arrays of micro-lenses using a sol gel glass replication technology is presented. Polydimethyl-siloxane (PDMS) master structures are used for the replication of thin glass membranes, which, by an appropriate annealing procedure, res ...

2002

Fabrication of multilayer systems combining microfluidic and micro-optical elements for fluorescence detection

Nico de Rooij, Hans Peter Herzig, René Dändliker

This paper presents the fabrication of a microchemical chip for the detection of fluorescence species in microfluidics. The microfluidic network is wet-etched in a Borofloat 33 (Pyrex) glass wafer and sealed by means of a second wafer. Unlike other similar ...

2001

Compact fast-steering tip/tilt laser scanner for high power material processing applications

Moustapha Hafez

The common configuration for deflecting laser beams in two dimensions is based on two single-axis galvanometric scanners placed perpendicular to each other. Over recent years, these fast scanners have proved to be both very reliable and very accurate. They ...

EPFL2000

Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms

Christian Depeursinge, Etienne Cuche, Pierre Marquet

We present a digital method for holographic microscopy involving a CCD camera as a recording device. Off-axis holograms recorded with a magnified image of microscopic objects are numerically reconstructed in amplitude and phase by calculation of scalar dif ...

1999

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