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Crypte de Lieberkühn
Publications associées (65)
Innovative Biomarkers for Obesity and Type 1 Diabetes Based on Bifidobacterium and Metabolomic Profiling
Stephan Morgenthaler, Paul Refinetti, Mariya Yuryevna Skvortsova
The role of Bifidobacterium species and microbial metabolites such as short-chain fatty acids (SCFAs) and human milk oligosaccharides in controlling intestinal inflammation and the pathogenesis of obesity and type 1 diabetes (T1D) has been largely studied ...
MDPI2024Engineering hydrogel microenvironments for epithelial organoid culture
Organoids, miniature tissues generated from self-organizing stem cells within three-dimensional (3D) extracellular matrices (ECM), have opened up exciting possibilities for in vitro studies of complex physiological processes. A key factor in the success of ...
EPFL2024Overexpression of human alpha-Synuclein leads to dysregulated microbiome/metabolites with ageing in a rat model of Parkinson disease
Hilal Lashuel, Florian Lang, Joan Romani Aumedes, Yogesh Singh
Background Braak's hypothesis states that sporadic Parkinson's disease (PD) follows a specific progression of pathology from the peripheral to the central nervous system, and this progression can be monitored by detecting the accumulation of alpha-Synuclei ...
BMC2023Microfluidic devices for automated analysis of ingested bacteria in the Caenorhabditis elegans intestine
In this thesis, we explored on-chip high-resolution imaging of the fate of intestinal bacteria and bacterial products in Caenorhabditis elegans (C. elegans). In the first part, we carried out high-resolution z-stack fluorescent imaging of Red Fluorescent P ...
EPFL2023Bioengineering human mini-intestines with in vivo-like complexity and function
Biological research heavily relies on the use of animal models, which has made it difficult to answer specific questions about human biology and disease. However, with the advent of human organoids - miniature versions of tissues generated in 3D human stem ...
EPFL2023Multiscale light-sheet organoid imaging framework
Petr Strnad, Andrea Boni, Raphaël Ortiz
Live imaging of organoid growth remains a challenge: it requires long-term imaging of several samples simultaneously and dedicated analysis pipelines. Here the authors report an experimental and image processing framework to turn long-term light-sheet imag ...
NATURE PORTFOLIO2022High-content, targeted RNA-seq screening in organoids for drug discovery in colorectal cancer
Maxim Norkin, Paloma Ordonez Moran
Organoids allow the recapitulation of intestinal homeostasis and cancerogenesis in vitro; however, RNA sequencing (RNA-seq)-based methods for drug screens are missing. We develop targeted organoid sequencing (TORNADO-seq), a high-throughput, high-content d ...
2021Intestinal organoid cocultures with microbes
This protocol comprises various methods to coculture organoids (particularly human small intestinal and colon organoids) with microbes, including microinjection into the lumen and periphery of 3D organoids and exposure of organoids to microbes in a 2D laye ...
NATURE PORTFOLIO2021Toxicity and translocation of Ag, CuO, ZnO and TiO2 nanoparticles upon exposure to fish intestinal epithelial cells
Understanding the ability of fish intestinal cells to act as a barrier for nanoparticle (NP) uptake and their effects is of significance from an environmental perspective as well as for human health, for which fish serves as an important nutrient source. W ...
ROYAL SOC CHEMISTRY2021Cytotoxicity, Accumulation and Translocation of Silver and Silver Sulfide Nanoparticles in contact with Rainbow Trout Intestinal Cells
Silver nanoparticles (Ag NPs) are widely used in consumer products especially because of their antimicrobial properties. However, this wide usage of Ag NPs is accompanied by their release into the environment where they will be rapidly transformed to other ...
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