Publications associées à Électrophorèse bidimensionnelle

Telomere protection against oxidative stress

Telomeres are nucleoprotein structures at the ends of linear chromosomes, being essential for the maintenance of genomic integrity. Telomeres have a unique structure which distinguishes chromosome termini from DNA damage sites. Shelterin complexes are the ...

EPFL2023

Site-Specific Protein Conjugation onto Fluorescent Single-Walled Carbon Nanotubes

Ardemis Anoush Boghossian, Shang-Jung Wu, Vitalijs Zubkovs, Nils Schürgers

Semiconducting single-walled carbon nanotubes (SWCNTs) are among the few photostable optical emitters that are ideal for sensing, imaging, drug delivery, and monitoring of protein activity. These applications often require strategies for immobilizing prote ...

2020

A smart low molecular weight gelator for the triple detection of copper (II), mercury (II), and cyanide ions in water resources

Rosario Scopelliti

Novel supramolecular gelators based on quinoline-indolin-2-one structure were synthesized. These gelators formed stable organogels in a mixture of DMSO/H2O (1:1). The scanning electron microscopy (SEM), IR and NMR spectroscopies, and rheological measuremen ...

ELSEVIER2020

Comparison of the functional properties of trimeric and monomeric CaiT of Escherichia coli

Kamila Krystyna Guérin

Secondary transporters exist as monomers, dimers or higher state oligomers. The significance of the oligomeric state is only partially understood. Here, the significance of the trimeric state of the L-carnitine/gamma-butyrobetaine antiporter CaiT of Escher ...

Nature Research2019

Equilibrium and Out-of-Equilibrium Adherence of Hydrogels against Polymer Brushes

Jennifer Mary Adrienne Alice Macron, Costantino Creton

At low pH, underwater adherence between poly(N,N-dimethylacrylamide) hydrogels and poly(acrylic acid) brushes is due to the formation of multiple hydrogen bonds. The effect of the key parameters controlling the formation of these interactions (contact time ...

American Chemical Society2018

Castles fall from inside: Evidence for dominant internal photo-catalytic mechanisms during treatment of Saccharomyces cerevisiae by photo-Fenton at near-neutral pH

César Pulgarin, Sami Rtimi, Stefanos Giannakis, Cristina del Socorro Lonfat

In this work, the antimicrobial effects of the photo-Fenton process on yeast cells were tested (Saccharomyces cerevisiae), an essential eukaryotic unicellular model of living cells. Near-neutral pH was used in all studies, while iron sulfate and iron citra ...

Elsevier2016

Investigation of cell death mechanisms in human lymphatic endothelial cells undergoing photodynamic therapy

Witold Waldemar Kilarski

Background: Photodynamic therapy (PDT) has been shown to induce ablation and functional occlusion of tumor-associated lymphatic vessels. However, direct effects of PDT on lymphatic endothelial cells (LECs) have not been studied so far. The aim of this stud ...

Elsevier Science Bv2016

Porous silica enhanced proteolysis during Off-Gel separation for efficient protein identification

Hubert Girault, Liang Qiao, Mohammad Reza Pourhaghighi

An advanced approach is developed in this work for simultaneous on-line separation and digestion of proteins by combining the Off-Gel isoelectric focusing (IEF) and enzymatic nanoreactor enhanced proteolysis. The nanoreactor was prepared by preloading tryp ...

Elsevier2015

Proteins/peptides purification by a three-well OFFGEL electrophoresis with immobilized ultra narrow pH gradient gels

Hubert Girault, Liang Qiao, Fernando Cortes Salazar, Elena Tobolkina

Purification and desalting of protein and peptide samples by a three-well OFFGEL electrophoresis with immobilized ultra narrow pH gradient gels is proposed as a fast preparative strategy for proteomics. The gist of this strategy is to separate the proteins ...

Royal Society of Chemistry2014

A four-component organogel based on orthogonal chemical interactions

Kay Severin, Kurt Schenk, Nicolas Luisier

A thermoresponsive organogel was obtained by orthogonal assembly of four compounds using dynamic covalent boronate ester and imine bonds, as well as dative boron-nitrogen bonds. It is shown that the gel state can be disrupted or reinforced by chemicals whi ...

Royal Society of Chemistry2014