Publications associées à Microinjection

Generation of a Gal4-dependent gene recombination and illuminating mouse

Cell labeling technologies, including the Cre/loxP system, are powerful tools in developmental biology. Although the conventional Cre/loxP system has been extensively used to label the expression of specific genes, it is less frequently used for labeling p ...

INT PRESS EDITING CENTRE INC2022

Intestinal organoid cocultures with microbes

Devanjali Dutta

This protocol comprises various methods to coculture organoids (particularly human small intestinal and colon organoids) with microbes, including microinjection into the lumen and periphery of 3D organoids and exposure of organoids to microbes in a 2D laye ...

NATURE PORTFOLIO2021

AFM Based Single Cell Microinjection : Technological Developements, Biological Experiments and Biophysical Analysis of Probe Indentation

Joanna Katarzyna Bitterli

The development of atomic force microscopy (AFM) has enabled a major breakthrough in the study of individual biological objects, such as nucleic acids, proteins and protein complexes. More recently the use of AFM to investigate eukaryotic cells has been ex ...

EPFL2012

Role of non-specific DNA in reducing coding DNA requirement for transient gene expression with CHO and HEK-293E cells

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Yashas Rajendra, Divor Kiseljak, Sagar Shashidhar Manoli

Transient gene expression (TGE) is a rapid method for the production of recombinant proteins in mammalian cells. While the TGE volumetric productivity has improved significantly over the past decade, the amount of plasmid DNA (pDNA) needed for transfection ...

2012

Studies on lentiviral-mediated transgenesis

Marc-Olivier Sauvain

Transgenic animals are essential research tools, whether to address basic biological questions or to develop preclinical models of human diseases. Their generation through the injection of naked plasmid DNA into the male pronucleus of a fertilized oocyte h ...

EPFL2009

Studies on scaffold design for tissue engineering applications : contribution of synthetic, natural and recombinant materials

The aim of tissue engineering is to regenerate tissue for the purpose of repairing or replacing diseased or injured tissue. Therefore, cells that are able to proliferate are seeded on scaffolds that provide mechanical stability and direct the three-dimensi ...

EPFL2009

Genotypic features of lentivirus transgenic mice

Didier Trono, Denis Duboule, Felix Naef, Marc-Olivier Sauvain, Frédéric Schütz

Lentivector-mediated transgenesis is increasingly used, whether for basic studies as an alternative to pronuclear injection of naked DNA or to test candidate gene therapy vectors. In an effort to characterize the genetic features of this approach, we first ...

American Society for Microbiology2008

Lentiviral vector-mediated genetic modification of human neural progenitor cells for ex vivo gene therapy

Patrick Aebischer, Bernard Schneider, Romain Zufferey

Human neural progenitor cells (hNPC) hold great potential as an ex vivo system for delivery of therapeutic proteins to the central nervous system. When cultured as aggregates, termed neurospheres, hNPC are capable of significant in vitro expansion. In the ...

2007

Generation of recombinant Chinese hamster ovary cell lines by microinjection

Florian Maria Wurm, Martin Jordan, Madiha Derouazi, Philippe Pierre Girard

Microinjection is a gene transfer technique enabling partial control of plasmid delivery into the nucleus or cytoplasm of cultured animal cells. Here this method was used to establish various recombinant mammalian cell lines. The injection volume was estim ...

Kluwer Academic Publishers, Springer2006

Establishment of recombinant mammalian cell lines : new approaches

Madiha Derouazi

Recombinant proteins are gaining in importance for therapeutic applications. The proteins are expressed in stable cell lines, within which recombinant DNA has incorporated into the host cells genome. Identification and isolation of extremely high producers ...

EPFL2005