Publications associées à ADN polymérase I

Towards an artificial cell: Development of a system which self-regenerates the protein components of the PURE system in microfluidic reactors

Thanks to recent advancements in synthetic biology, the dream of creating a synthetic cell has become feasible. However, due to its inherent complexity, one of the fundamental functions of all living systems, i.e., self-replication, remains to be introduce ...

EPFL2021

A partially self-regenerating synthetic cell

Sebastian Maerkl, Barbora Lavickova, Nadanai Laohakunakorn

Self-regeneration is a fundamental function of all living systems. Here we demonstrate partial molecular self-regeneration in a synthetic cell. By implementing a minimal transcription-translation system within microfluidic reactors, the system is able to r ...

Nature Research2020

Isothermal multiple displacement amplification of DNA templates in minimally buffered conditions using phi29 polymerase

Carlotta Guiducci, Enrico Tenaglia

The isothermal amplification of DNA in minimally buffered conditions allows to perform and monitor nucleic acid amplification with minimal technological and operative requirements. We show in this work how phi29 can operate multiple displacement amplificat ...

Royal Society of Chemistry2018

Extraction of cell-free DNA from urine, using polylysine-coated silica particles

Paul Refinetti, Sho Takano

DNA analysis is used for a variety of purposes, including disease diagnosis and DNA profiling; this involves extracting DNA from living organisms. In this study, we prepared polycationic silica particles to extract DNA that has the negatively charged phosp ...

Springer Heidelberg2017

Cooperative motion of a key positively charged residue and metal ions for DNA replication catalyzed by human DNA Polymerase-eta

Matteo Dal Peraro, Vito Genna

Trans-lesion synthesis polymerases, like DNA Polymerase-eta (Pol-eta), are essential for cell survival. Pol-eta bypasses ultraviolet-induced DNA damages via a two-metal-ion mechanism that assures DNA strand elongation, with formation of the leaving group p ...

Oxford University Press (OUP)2016

On the statistical physics of chains and rods, with application to multi-scale sequence-dependent DNA modelling

Alexandre Emmanuel Grandchamp

The complex mechanisms involved in cellular processes have been increasingly understood this past century and the central role of the DNA molecule has been recognized. The base pair sequence along a DNA fragment is observed not only to encode the genomic i ...

EPFL2016

Upstream Binding of Idling RNA Polymerase Modulates Transcription Initiation from a Nearby Promoter

Giovanni Dietler, Aleksandre Japaridze

The bacterial gene regulatory regions often demonstrate distinctly organized arrays of RNA polymerase binding sites of illdefined function. Previously we observed a module of closely spaced polymerase binding sites upstream of the canonical promoter of the ...

American Society for Biochemistry and Molecular Biology2015

CST for the grand finale of telomere replication

Joachim Lingner, Liuh-Yow Chen

Telomeric DNA at eukaryotic chromosome ends terminates with single stranded 3' G-rich overhangs. The overhang is generated by the interplay of several dynamic processes including semiconservative DNA replication, 3' end elongation by telomerase, C-strand f ...

Landes Bioscience2013

Molecular basis of telomere syndrome caused by CTC1 mutations

Joachim Lingner, Liuh-Yow Chen, Jana Majerská

Mutations in CTC1 lead to the telomere syndromes Coats Plus and dyskeratosis congenita (DC), but the molecular mechanisms involved remain unknown. CTC1 forms with STN1 and TEN1 a trimeric complex termed CST, which binds ssDNA, promotes telomere DNA synthes ...

Cold Spring Harbor Laboratory Press2013

Cooperative kinking at distant sites in mechanically stressed DNA

Davide Demurtas, Guillaume Witz, Julien Dorier, Andrzej Stasiak

In cells, DNA is routinely subjected to significant levels of bending and twisting. In some cases, such as under physiological levels of supercoiling, DNA can be so highly strained, that it transitions into non-canonical structural conformations that are c ...

Oxford University Press2011