Concept
ADN polymérase I
Publications associées (42)
Interplay of the mechanical and structural properties of DNA nanostructures determines their electrostatic interactions with lipid membranes
Maartje Martina Cornelia Bastings, Cem Tekin, Diana Kornelia Morzy, Vincenzo Caroprese
Nucleic acids and lipids function in close proximity in biological processes, as well as in nanoengineered constructs for therapeutic applications. As both molecules carry a rich charge profile, and frequently coexist in complex ionic solutions, the electr ...
ROYAL SOC CHEMISTRY2023Polydimethylsiloxane microstructure-induced acoustic streaming for enhanced ultrasonic DNA fragmentation on a microfluidic chip
Martinus Gijs, Thomas Lehnert, Lin Sun
Next-generation sequencing (NGS) is an essential technology for DNA identification in genomic research. DNA fragmentation is a critical step for NGS and doing this on-chip is of great interest for future integrated genomic solutions. Here we demonstrate fa ...
ROYAL SOC CHEMISTRY2022DNA-Liposome Hybrid Carriers for Triggered Cargo Release
The design of simple and versatile synthetic routes to accomplish triggered-release properties in carriers is of particular interest for drug delivery purposes. In this context, the programmability and adaptability of DNA nanoarchitectures in combination w ...
AMER CHEMICAL SOC2022Towards an artificial cell: Development of a system which self-regenerates the protein components of the PURE system in microfluidic reactors
Thanks to recent advancements in synthetic biology, the dream of creating a synthetic cell has become feasible. However, due to its inherent complexity, one of the fundamental functions of all living systems, i.e., self-replication, remains to be introduce ...
EPFL2021The more the merrier: how homo-oligomerization alters the interactome and function of ribonucleotide reductase
Stereotyped as a nexus of dNTP synthesis, the dual-subunit enzyme - ribonucleotide reductase (RNR) - is coming into view as a paradigm of oligomerization and moonlighting behavior. In the present issue of `omics', we discuss what makes the larger subunit o ...
ELSEVIER SCI LTD2020Isothermal multiple displacement amplification of DNA templates in minimally buffered conditions using phi29 polymerase
Carlotta Guiducci, Enrico Tenaglia
The isothermal amplification of DNA in minimally buffered conditions allows to perform and monitor nucleic acid amplification with minimal technological and operative requirements. We show in this work how phi29 can operate multiple displacement amplificat ...
2018Extraction of cell-free DNA from urine, using polylysine-coated silica particles
DNA analysis is used for a variety of purposes, including disease diagnosis and DNA profiling; this involves extracting DNA from living organisms. In this study, we prepared polycationic silica particles to extract DNA that has the negatively charged phosp ...
Springer Heidelberg2017Cooperative motion of a key positively charged residue and metal ions for DNA replication catalyzed by human DNA Polymerase-eta
Trans-lesion synthesis polymerases, like DNA Polymerase-eta (Pol-eta), are essential for cell survival. Pol-eta bypasses ultraviolet-induced DNA damages via a two-metal-ion mechanism that assures DNA strand elongation, with formation of the leaving group p ...
Oxford University Press (OUP)2016On the statistical physics of chains and rods, with application to multi-scale sequence-dependent DNA modelling
The complex mechanisms involved in cellular processes have been increasingly understood this past century and the central role of the DNA molecule has been recognized. The base pair sequence along a DNA fragment is observed not only to encode the genomic i ...
EPFL2016