Concept
Fragment d'Okazaki
Publications associées (46)
Regulation and impact of TERRA R-loops at human telomeres
Telomeres are the nucleoprotein structures found at the ends of linear chromosomes. They ensure that the termini of chromosomes are not inappropriately recognized as sites of DNA damage, and are therefore crucial for genome stability. In spite of the heter ...
EPFL2023The THO complex counteracts TERRA R-loop-mediated telomere fragility in telomerase(+) cells and telomeric recombination in ALT(+) cells
Joachim Lingner, Rita Valador Fernandes
Telomeres are the nucleoprotein structures at the ends of linear chromosomes. Telomeres are transcribed into long non-coding Telomeric Repeat-Containing RNA (TERRA), whose functions rely on its ability to associate with telomeric chromatin. The conserved T ...
OXFORD UNIV PRESS2023Polydimethylsiloxane microstructure-induced acoustic streaming for enhanced ultrasonic DNA fragmentation on a microfluidic chip
Martinus Gijs, Thomas Lehnert, Lin Sun
Next-generation sequencing (NGS) is an essential technology for DNA identification in genomic research. DNA fragmentation is a critical step for NGS and doing this on-chip is of great interest for future integrated genomic solutions. Here we demonstrate fa ...
ROYAL SOC CHEMISTRY2022Identification and characterization of proteins that protect human telomeres from fragility
Difficulties to replicate telomeres - the ends of our chromosomes - can cause telomere shortening andgenome instability. These difficulties are due to the repetitive DNA sequence and distinct structures at telomeresthat challenge the semi-conservative DNA ...
EPFL2022Challenging endings: How telomeres prevent fragility
It has become apparent that difficulties to replicate telomeres concern not only the very ends of eukaryotic chromosomes. The challenges already start when the replication fork enters the telomeric repeats. The obstacles encountered consist mainly of nonca ...
WILEY2021The human telomeric proteome during telomere replication
Joachim Lingner, Thomas Frédéric Lunardi, Aleksandra Vancevska, Gérald Joseph Paul Lossaint, Chih-Yi Gabriela Lin
Telomere shortening can cause detrimental diseases and contribute to aging. It occurs due to the end replication problem in cells lacking telomerase. Furthermore, recent studies revealed that telomere shortening can be attributed to difficulties of the sem ...
OXFORD UNIV PRESS2021Redox Properties of Native and Damaged DNA from Mixed Quantum Mechanical/Molecular Mechanics Molecular Dynamics Simulations
Ursula Röthlisberger, Ivano Tavernelli, Polydefkis Diamantis
The redox properties of two large DNA fragments composed of 39 base pairs, differing only by an 8-oxoguanine (8oxoG) defect replacing a guanine (G), were investigated in physiological conditions using mixed quantum mechanical/molecular mechanical (QM/MM) m ...
AMER CHEMICAL SOC2020Human shelterin protein POT1 prevents severe telomere instability induced by homology-directed DNA repair
Joachim Lingner, Anna-Sophia Briod
The evolutionarily conserved POT1 protein binds single-stranded G-rich telomeric DNA and has been implicated in contributing to telomeric DNA maintenance and the suppression of DNA damage checkpoint signaling. Here, we explore human POT1 function through g ...
WILEY2020Generation of a Large Peptide Phage Display Library by Self-Ligation of Whole-Plasmid PCR Product
Christian Heinis, Xudong Kong, Vanessa Carle, Cristina Diaz Perlas, Kaycie Marie Butler
The success of phage display, used for developing target-specific binders based on peptides and proteins, depends on the size and diversity of the library screened, but generating large libraries of phage-encoded polypeptides remains challenging. New pepti ...
2020PaRiS: Causally Consistent Transactions with Non-blocking Reads and Partial Replication
Willy Zwaenepoel, Diego Didona, Kristina Spirovska
Geo-replicated data platforms are at the backbone of several large-scale online services. Transactional Causal Consistency (TCC) is an attractive consistency level for building such platforms. TCC avoids many anomalies of eventual consistency, eschews the ...
2019