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Affinity precipitation was compared to affinity chromatography and batch adsorption as the final purification step in a protocol for the isolation of haemoglobin from human blood. Haptoglobin was the affinity ligand. The first steps on the process were rea ...
Product isolation increasingly becomes a major bottleneck in molecular biotechnology. This is especially the case for the sensitive proteineous substances (recombinant proteins, antibodies) and lately also DNA, usually produced in low concentration. The cu ...
The interactions and structural formation of polyelectrolytes in solution and environmental stimuli-responsive water-soluble polymers are subjects of intense academic research, development, and application. An increasing number of novel materials are being ...
Nanoparticles are finding many research and industrial applications, yet their characterization remains a challenge. Their cores are often polydisperse and coated by a stabilizing shell that varies in size and composition. No single technique can character ...
Centrifuge experiments modeling single-phase flow in prototype porous media typically use the same porous medium and permeant. Then, well-known scaling laws are used to transfer the results to the prototype. More general scaling laws that relax these restr ...
Unstable flow can result in the formation of fingers during infiltration into dry soil. Centrifuge modeling is a potentially useful tool to study the relationship between finger size, spacing, and velocity. It can also be used to investigate solute transpo ...
A variety of extracellular ligands and pathogens interact with raft domains in the plasma membrane of eukaryotic cells. In this study, we examined the role of lipid rafts and raft-associated glycosylphosphatidylinositol (GPI)-anchored proteins in the proce ...
This paper reports on the dilute, aqueous solution self-assembly of two asymmetric polystyrene-b-poly(L-lysine) (PSnPKm) diblock oligomers with PS and PK number-average degrees of polymerization of eight, respectively, 40 and 60. The self-assembly of these ...
The concept of “mobile” and “immobile” fluid regions (as incorporated in the Two-Region Model, TRM) is often used in the governing equation to model tailing in solute breakthrough curves (BTC). In this paper, we apply the TRM and its parent equation, the A ...
The growing demand for high levels of recombinant proteins of medical and pharmaceutical interest stimulates the development of cell bioprocess technology. Spin-filter technology is employed in order to reach high levels of such compounds. The aim of this ...
