Publications associées à Fluorescéine

DNA-Liposome Hybrid Carriers for Triggered Cargo Release

The design of simple and versatile synthetic routes to accomplish triggered-release properties in carriers is of particular interest for drug delivery purposes. In this context, the programmability and adaptability of DNA nanoarchitectures in combination w ...

AMER CHEMICAL SOC2022

A 512 x 512 SPAD Image Sensor With Integrated Gating for Widefield FLIM

Edoardo Charbon, Claudio Bruschini, Ivan Michel Antolovic, Arin Can Ülkü

In this paper, we report on SwissSPAD2, an image sensor with 512 x 512 photon-counting pixels, each comprising a single-photon avalanche diode (SPAD), a 1-b memory, and a gating mechanism capable of turning the SPAD ON and OFF, with a skew of 250 and 344 p ...

IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC2019

Cellular Uptake and Intracellular Trafficking of Poly(N-(2-Hydroxypropyl) Methacrylamide)

Anders Meibom, Harm-Anton Klok, Graham Knott, Stéphane Laurent Escrig, Olivier Burri, Arne Seitz, Claudia Battistella, Romain Guiet

Cellular uptake and intracellular trafficking of polymer conjugates or polymer nanoparticles is typically monitored using fluorescence-based techniques such as confocal microscopy. While these methods have provided a wealth of insight into the internalizat ...

American Chemical Society2019

In vivo pulse labeling of isochronic cohorts of cells in the central nervous system using FlashTag

Subashika Govindan

The tracing of neuronal cell lineages is critical to our understanding of cellular diversity in the CNS. This protocol describes a fluorescence birth-dating technique to label, track and isolate isochronic cohorts of newborn cells in the CNS in vivo in mou ...

NATURE PUBLISHING GROUP2018

Preclinical Evaluation of Photoacoustic Imaging as a Novel Noninvasive Approach to Detect an Orthopaedic Implant Infection

Riccardo Sinisi, Pavlo Khodakivskyi, Ghyslain Martial Alain Budin

Introduction: Diagnosing-prosthetic joint infection (PJI) poses significant challenges, and current modalities are fraught with low sensitivity and/or potential morbidity. Photoacoustic imaging (PAI) is a novel ultrasound-based modality with potential for ...

Lippincott Williams & Wilkins2017

Bulk velocity measurements by video analysis of dye tracer in a macro-rough channel

Anton Schleiss, Mário Jorge Rodrigues Pereira Da Franca, Tamara Ghilardi

Steep mountain rivers have hydraulic and morphodynamic characteristics that hinder velocity measurements. The high spatial variability of hydraulic parameters, such as water depth (WD), river width and flow velocity, makes the choice of a representative cr ...

Institute of Physics2014

Reduced Dyes Enhance Single-Molecule Localization Density for Live Superresolution Imaging

Suliana Manley, Luc Reymond, Aleksandr Benke, Grazvydas Lukinavicius, Lina Carlini

Cell-permeable rhodamine dyes are reductively quenched by NaBH4 into a non-fluorescent leuco-rhodamine form. Quenching is reversible, and their fluorescence is recovered when the dyes are oxidized. In living cells, oxidation occurs spontaneously, and can r ...

Wiley-V C H Verlag Gmbh2014

Time-resolved imaging system for fluorescence-guided surgery with lifetime imaging capability

Edoardo Charbon, Claudio Bruschini, Douglas Hanahan, François Eric Powolny, Harald Arjan Robert Homulle, Krisztian Homicsko, Riccardo Sinisi

We present a single-photon camera for fluorescence imaging, with a time resolution better than 100ps, capable of providing both intensity and lifetime images. the camera was fabricated in standard CMOS technology. With this FluoCam we show the possibility ...

Spie-Int Soc Optical Engineering2014

Photobleaching Kinetics and Time-Integrated Emission of Fluorescent Probes in Cellular Membranes

Daniel Sage

Since the pioneering work of Hirschfeld, it is known that time-integrated emission (TiEm) of a fluorophore is independent of fluorescence quantum yield and illumination intensity. Practical implementation of this important result for determining exact prob ...

Mdpi Ag2014

A 2D/3D image analysis system to track fluorescently labeled structures in rod-shaped cells: application to measure spindle pole asymmetry during mitosis

Michaël Unser, Daniel Sage, Viesturs Simanis, Daniel Andreas Schmitter, Anastasia Chasapi, Ioannis Xenarios

Background: The yeast Schizosaccharomyces pombe is frequently used as a model for studying the cell cycle. The cells are rod-shaped and divide by medial fission. The process of cell division, or cytokinesis, is controlled by a network of signaling proteins ...

Biomed Central Ltd2013