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For many metabolic systems, available exptl. data allow description of the system by elasticities and control coeffs. The availability of information of this kind motivated the development of a (log)linear kinetic model of metabolic systems that is complet ...
In this paper, we have investigated the effects of the pore-forming toxin aerolysin, produced by Aeromonas hydrophila, on mammalian cells. Our data indicate that the protoxin binds to an 80-kD glycosyl-phosphatidylinositol (GPI)-anchored protein on BHK cel ...
Many attempts to engineer cellular metab. have failed due to the complexity of cellular functions. Math. and computational methods are needed that can organize the available exptl. information, and provide insight and guidance for successful metabolic engi ...
Body mass and composition reflect the combined effects of three processes: energy intake, energy partitioning (storage), and energy expenditure. Energy is released from food as it is combusted to carbon dioxide and water, and is expended as heat and work w ...
The objective of this study was to characterize the dynamic adaptation of the oxidative capacity of Saccharomyces cerevisiae to an increase in the glucose supply rate and its implications for the control of a continuous culture designed to produce biomass ...
To meet the need for studies of anaerobic microbial and animal cell cultures involving much lower heat effects as compared to aerobic microbial cultures, a bench scale calorimeter, Bio-RCl, has been improved for achieving a higher long-term sensitivity. Th ...
Aerobic chemostat cultures of Saccharomyces cerevisiae were performed under carbon-, nitrogen-, and dual carbon- and nitrogen-limiting conditions. The glucose concn. was kept const., whereas the ammonium concn. was varied among different expts. and differe ...
Aerobic chemostat cultures of S. cerevisiae were performed at different diln. rates under energy (glucose) limitation or at various extents of energy excess imposed by a N limitation. Energy excess induced metabolic uncoupling under steady-state conditions ...
A review with many refs. The recent development of two math. frameworks for the anal. and design of metabolic systems is reviewed here with many refs. Both frameworks employ a (log)linear kinetic metabolic model that is constructed making direct, explicit ...
A review with 29 refs. of lab.-scale investigations carried out on the usefulness of biol. heat-release measurements, as a means for monitoring and controlling the metabolic state of microbial cultures. Such studies are carried out in high-quality bench-sc ...
