Publications associées à Inhibiteur enzymatique

Directed evolution of the suicide protein O⁶-alkylguanine-DNA alkyltransferase for increased reactivity results in an alkylated protein with exceptional stability

Ursula Röthlisberger, Kai Johnsson, Florence Pojer, Elizabeth Claire Brunk, Birgit Mollwitz, Simone Schmitt

Here we present a biophysical, structural, and computational analysis of the directed evolution of the human DNA repair protein O-6-alkylguanine-DNA alkyltransferase (hAGT) into SNAP-tag, a self-labeling protein tag. Evolution of hAGT led not only to incre ...

2012

Structure and function of the transketolase from Mycobacterium tuberculosis and comparison with the human enzyme

Stewart Cole, Florence Pojer, Elizabeth Fullam

The transketolase (TKT) enzyme in Mycobacterium tuberculosis represents a novel drug target for tuberculosis treatment and has low homology with the orthologous human enzyme. Here, we report on the structural and kinetic characterization of the transketola ...

Royal Soc2012

Functional diversity of reductive dehalogenases in a bacterial consortium degrading chloroethenes

Christof Holliger, Julien Maillard, Géraldine Florence Buttet

Several enrichment cultures were investigated in our laboratory for the bioremediation potential of tetrachloroethene (PCE). The underlying process, organohalide respiration (OHR), is based on bacterial anaerobic respiration in which the chlorinated compou ...

2012

Determination of optimal conditions for the degradation of micropollutants by laccase from Trametes versicolor

David Andrew Barry, Christof Holliger, Julien Maillard, Luca Rossi, Jonas Margot

Many hydrophilic organic compounds present at low concentrations in municipal wastewater, such as several pharmaceuticals, biocides and pesticides, are recalcitrant in conventional wastewater treatment plants. To improve the biodegradation of these compoun ...

2012

Towards a new tuberculosis drug: pyridomycin - nature's isoniazid

Stewart Cole, Florence Pojer, Claudia Sala, Ruben Hartkoorn, Joao Pedro De Almeida Neres, Swapna Uplekar, Raju Mukherjee, Sophie Magnet

Tuberculosis, a global threat to public health, is becoming untreatable due to widespread drug resistance to frontline drugs such as the InhA-inhibitor isoniazid. Historically, by inhibiting highly vulnerable targets, natural products have been an importan ...

Wiley Open Access2012

Role of Environment for Catalysis of the DNA Repair Enzyme MutY

Ursula Röthlisberger, Jeremy Samuel Arey, Elizabeth Claire Brunk

Control of the N-glycosylase reaction by the DNA repair enzyme, MutY, entails the organization of solvent molecules. Classical molecular dynamics and QM/MM simulations were used to investigate the solvent and environment effects contributing to catalysis. ...

2012

Functional diversity of reductive dehalogenases in a bacterial consortium degrading chloroethenes

Christof Holliger, Julien Maillard, Géraldine Florence Buttet

Enrichment cultures are investigated for the bioremediation potential of tetrachloroethene (PCE). This process is based on anaerobic respiration in which chlorinated compounds are used as electron acceptors (organohalide respiration, OHR). The key enzyme i ...

2012

Fluorescence based low-cost microbiosensors using simultaneously microfabrication process and enzyme immobilisation

Nico de Rooij, Peter Van der Wal, Sotiria Psoma

A novel one-step microfabrication process of SU-8 films is proposed for developing inexpensive, small size and light-weight optical glucose micro-biosensors. Experimental work was performed in order to investigate whether the widely used in MEMS applicatio ...

2012

Structural Basis for Benzothiazinone-Mediated Killing of Mycobacterium tuberculosis

Paul Joseph Dyson, Stewart Cole, John McKinney, Neeraj Dhar, Florence Pojer, Joao Pedro De Almeida Neres, Elizabeth Fullam

The benzothiazinone BTZ043 is a tuberculosis drug candidate with nanomolar whole-cell activity. BTZ043 targets the DprE1 catalytic component of the essential enzyme decaprenylphosphoryl-beta-D-ribofuranose-2'-epimerase, thus blocking biosynthesis of arabin ...

American Association for the Advancement of Science2012

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Inhibiteur enzymatique

Directed evolution of the suicide protein O⁶-alkylguanine-DNA alkyltransferase for increased reactivity results in an alkylated protein with exceptional stability

Structure and function of the transketolase from Mycobacterium tuberculosis and comparison with the human enzyme

Functional diversity of reductive dehalogenases in a bacterial consortium degrading chloroethenes

Determination of optimal conditions for the degradation of micropollutants by laccase from Trametes versicolor

Towards a new tuberculosis drug: pyridomycin - nature's isoniazid

Impact of virus aggregation on disinfection

Role of Environment for Catalysis of the DNA Repair Enzyme MutY

Functional diversity of reductive dehalogenases in a bacterial consortium degrading chloroethenes

Fluorescence based low-cost microbiosensors using simultaneously microfabrication process and enzyme immobilisation

Structural Basis for Benzothiazinone-Mediated Killing of Mycobacterium tuberculosis

Graph Chatbot

Chattez avec Graph Search

Structure and function of the transketolase from Mycobacterium tuberculosis and comparison with the human enzyme

Functional diversity of reductive dehalogenases in a bacterial consortium degrading chloroethenes

Role of Environment for Catalysis of the DNA Repair Enzyme MutY

Impact of virus aggregation on disinfection

Fluorescence based low-cost microbiosensors using simultaneously microfabrication process and enzyme immobilisation

Structural Basis for Benzothiazinone-Mediated Killing of Mycobacterium tuberculosis

Determination of optimal conditions for the degradation of micropollutants by laccase from Trametes versicolor

Directed evolution of the suicide protein O⁶-alkylguanine-DNA alkyltransferase for increased reactivity results in an alkylated protein with exceptional stability

Functional diversity of reductive dehalogenases in a bacterial consortium degrading chloroethenes

Towards a new tuberculosis drug: pyridomycin - nature's isoniazid