Concept
Mutagénèse dirigée
Publications associées (99)
Molecular mechanism of plasmid elimination by the DdmDE defense system
Melanie Blokesch, Sandrine Stutzmann, David William Adams, Laurie Righi
Seventh pandemic Vibrio cholerae strains contain two pathogenicity islands that encode the DNA defense modules DdmABC and DdmDE. Here we use cryogenic electron microscopy to reveal the mechanistic basis for plasmid defense by DdmDE. A structure of the DdmD ...
2024Site-selective template-directed synthesis of antibody Fc conjugates with concomitant ligand release
Natalia Gasilova, Jean-Manuel Segura, David Viertl
Template-directed methods are emerging as some of the most effective means to conjugate payloads at selective sites of monoclonal antibodies (mAbs). We have previously reported a method based on an engineered Fc-III reactive peptide to conjugate a radionuc ...
Cambridge2023How Vibrio cholerae adapts to the environment: from cell shape transitions to antagonistic behavior
The bacterium Vibrio cholerae is the causative agent of the diarrheal disease cholera, which affects millions of people every year. Apart from being a human pathogen, V. cholerae is also a common member of aquatic habitats. Whilst the mechanism that allow ...
EPFL2022