Concept

Transformation (génétique)

Publications associées (124)

Transfection of partially purified plasmid DNA for high level transient protein expression in HEK293-EBNA cells

Florian Maria Wurm, Martin Jordan, Jason Wright

One of the major constraints to performing large-scale transfections of cultured mammalian cells for the transient expression of recombinant proteins is the production of large quantities of purified plasmid DNA. In this report partially purified plasmid D ...

2003

A computational framework for the discovery of novel biotransformations

Vassily Hatzimanikatis

The large no. of chem. transformations involved in biochem. pathways and the even larger no. of enzymes that catalyze these transformations contribute to the complexity of biol. systems. Anal. of the available genome sequences, as well as results from prio ...

2003

Transfection using plasmid preparations

Florian Maria Wurm, Jason Wright

There is provided a method for plasmid DNA extraction to reproducibly yield relatively crude preparation which is suitable for transfection into mammalian cells. The inventors have determined that the high level of purification previously thought to be nec ...

2002

Detection of promoter activity by flow cytometric analysis of GFP reporter expression

Joachim Lingner

Low efficiency of transfection is often the limiting factor for acquiring conclusive data in reporter assays. It is especially difficult to efficiently transfect and characterize promoters in primary human cells. To overcome this problem we have developed ...

2002

100-liter transient transfection

Florian Maria Wurm, Madiha Derouazi, Martin Jordan, Philippe Pierre Girard

This is the first report of two successful 100 l scale transienttransfections in a standard stirred bioreactor. More than half a gram of a monoclonal antibody (IgG) were produced in less than 10 days using a technology called large-scale transient gene exp ...

2002

Transfer of high copy number plasmid into mammalian cells by calcium phosphate transfection

Florian Maria Wurm, Martin Jordan

Using flow cytometry, single cell sorting, confocal microscopy and fluorescent plasmids, a thorough study of DNA uptake, DNA fate and DNA expression in mammalian cells transfected with the widely used calcium-phosphate precipitation method was executed. We ...

2001

Transient gene expression: recombinant protein production with suspension-adapted HEK293-EBNA cells

Florian Maria Wurm, Horst Pick, Klaus Friedrich

Transient gene expression (TGE) in mammalian cells at the reactor scale is becoming increasingly important for the rapid production of recombinant proteins. We improved a process for transient calcium phosphate-based transfection of HEK293-EBNA cells in a ...

2001

The physiology of the lymphatic system

Melody Swartz

This paper presents an overview of the anatomy, physiology, and biology of the lymphatic system specifically relevant to lymphatic drug delivery. We will briefly review the classic fluid and solute transport literature, and also examine the current researc ...

2001

Methods and materials for the synthesis of organic products

Vassily Hatzimanikatis

The invention provides methods and materials related to the prodn. of org. products. Specifically, the invention provides various recombinant yeast cells, methods for culturing yeast cells, methods for making yeast cells, nucleic acid constructs, and metho ...

2000

Too much interference: Injection of double-stranded RNA has nonspecific effects in the zebrafish embryo

Andrew Charles Oates

We have investigated the ability of double-stranded RNA (dsRNA) to inhibit gene expression in a vertebrate, the zebrafish, Danio rerio. Injection of dsRNA corresponding to the T-box gene tbx16/spadetail (spt) into early wild-type embryos caused a rapid and ...

2000

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