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The prodn. of acetate from the fermn. of lactate by Gluconobacter oxydans was studied. Batch expts. showed that glucose was the preferred substrate compared to lactate. A fed-batch culture was fed with a mixt. of glucose and lactate followed by periodic ad ...
Chemostat cultures of an aerobic fermenting yeast (Saccharomyces cerevisiae) and an aerobic respiring yeast (Kluyveromyces fragilis) were grown under dual C + N limitation in an isothermal reaction calorimeter. The dual limitations resulted in uncoupled ox ...
Yeast strains were examd. for their ability to produce glycerol, and Kluyveromyces marxianus NRRLY-665 was chosen for further optimization due to its comparatively high growth rate (0.15/h) and glycerol yield (9.5% by wt. on lactose) at 37 Deg and pH 7. Op ...
Heat generation is a very conspicuous phenomenon in large scale bioreactors and could in principle quite easily be measured by the temp. increase of the cooling water. Taking yeast cultures growing on a mixed oxido-reductive metab. for various reasons as a ...
The whi2 mutation in S. cerevisiae allows mutant cells to grow with a higher specific rate and at smaller cell sizes at the onset of EtOH metab. in diauxic growth. S. cerevisiae Whi2+ and whi2 were cultivated in aerobic and anaerobic batch cultures in the ...
A simple definition of the energetic growth efficiency both in terms of enthalpy and free energy is given and discussed. In cultures growing by respiratory catabolism, these efficiencies may conveniently be obsd. online by isothermal reaction calorimetry. ...
Measurements of the heat produced by Saccharomyces cerevisiae CBS 426 were used in conjunction with elemental and enthalpic balances to control fed-batch fermns. A proportional control using the ratio of carbon dioxide evolution rate to heat prodn. resulte ...
During the anaerobic batch cultivation of Kluyveromyces fragilis on a complex medium contg. glucose (100 g/L), EtOH was produced at levels resulting in the inhibition of growth and EtOH formation. Continuous extn. of the EtOH using transmembrane distn. res ...
The effect of pH on growth and lactic acid prodn. of L. helveticus was investigated in a continuous culture using supplemented whey ultrafiltrate. Max. lactate productivity of 5 g/L/h occurred at pH 5.5. Whey permeates concd. up to 4 times were fermented u ...
The heat generated by both batch and continuous cultures of the yeast Kluyveromycs fragilis was studied using a modified bench scale calorimeter. Batch cultures were used to measure the heat dissipation rates and the heat yields during fully aerobic and co ...