Concept
Gene delivery
Publications associées (195)
Comparative Evaluation of Lipofectamine and Dendrimer for Transfection of Short RNA Into Human T47D and MCF-10A Cell Lines
Purpose: Non-viral transfection approaches are extensively used in cancer therapy. The future of cancer therapy lies on targeted and efficient drug/gene delivery. The aim of this study was to determine the transfection yields of two commercially available ...
2023How are cells instructed to form somite boundaries by the zebrafish segmentation clock?
In vertebrate embryos, the elongating body axis is patterned via the sequential and rhyth-mic production of segments from a posterior unsegmented tissue called the presomitic mesoderm (PSM). This process is controlled by a population of cellular oscillator ...
EPFL2023Core-shell Structured Chitosan-polyethylenimine Nanoparticles for Gene Delivery: Improved Stability, Cellular Uptake, and Transfection Efficiency
Sandrine Gerber, Perrine Agnes Edith Robin, Laura Camille Louise Nicolle
Gene therapy emerged as a promising treatment option for acquired and inherited diseases. The delivery of nucleic acids relies on vectors that condense and encapsulate their cargo. Especially non-viral gene delivery systems are of increasing interest. Howe ...
2022Engineering synthetic adhesins for biophysical investigation and rewiring of host-microbe interactions
Host attachment is often a critical step in the onset of pathogenesis. To attach to host cells, bacteria have evolved a range of adhesins that bind to specific receptors. Some of these adhesins have been thoroughly characterized using biochemical technique ...
EPFL2022Engineering of chitosan-based derivatives for non-viral gene delivery
Gene therapy offers the possibility to treat or even cure diseases originating from genetic defects by introducing a therapeutic gene in target cells or correcting the initial defective gene. Amongst different options, non-viral vectors rely on the deliver ...
EPFL2022Development of Covalent Chitosan-Polyethylenimine Derivatives as Gene Delivery Vehicle: Synthesis, Characterization, and Evaluation
Sandrine Gerber, Céline Marie Anne Journot, Laura Camille Louise Nicolle
There is an increasing interest in cationic polymers as important constituents of non-viral gene delivery vectors. In the present study, we developed a versatile synthetic route for the production of covalent polymeric conjugates consisting of water-solubl ...
2021Biotechnology Applications of Nanocarbons in Plant and Algal Systems
Ardemis Anoush Boghossian, Alice Judith Gillen, Alessandra Antonucci
The unique combination of structural, optical, and electronic properties of nanocarbons (NCs) has made these materials particularly attractive for various applications in biotechnology and biomedicine, ranging from sensing, gene-delivery, bioimaging and th ...
Royal Society of Chemistry2021Efficient viral transduction in mouse inner ear hair cells with utricle injection and AAV9-PHP.B
Bernard Schneider, Paola Andrea Solanes Vega
Viral delivery of exogenous coding sequences into the inner ear has the potential for therapeutic benefit for patients suffering genetic or acquired hearing loss. To devise improved strategies for viral delivery, we investigated two injection techniques, r ...
2020Selection of Water-Soluble Chitosan by Microwave-Assisted Degradation and pH-Controlled Precipitation
Sandrine Gerber, Yann Lavanchy, Céline Marie Anne Journot, Laura Camille Louise Nicolle
In the field of gene therapy, chitosan (CS) gained interest for its promise as a non-viral DNA vector. However, commercial sources of CS lack precise characterization and do not generally reach sufficient solubility in aqueous media for in vitro and in viv ...
2020