Concept

Déficit immunitaire primitif

Publications associées (71)

Droplet-based microfluidics in drug discovery, transcriptomics and high-throughput molecular genetics

Droplet-based microfluidics enables assays to be carried out at very high throughput (up to thousands of samples per second) and enables researchers to work with very limited material, such as primary cells, patient's biopsies or expensive reagents. An add ...

2016

Microfluidic co-culture platform to quantify chemotaxis of primary stem cells

Zuzana Tatárová

Functional analysis of primary tissue-specific stem cells is hampered by their rarity. Here we describe a greatly miniaturized microfluidic device for the multiplexed, quantitative analysis of the chemotactic properties of primary, bone marrow-derived mese ...

Royal Soc Chemistry2016

Development of microfluidic devices for chemotaxis of primary stem cells

Zuzana Tatárová

Functional analysis of primary tissue-specific stem cells is hampered by their rarity. Here I developed greatly miniaturized microfluidic devices for the multiplexed, quantitative analysis of the chemotactic properties of primary, bone marrow-derived mesen ...

EPFL2016

Biomechanical forces promote blood development through prostaglandin E2 and the cAMP-PKA signaling axis

Olaia Maria Naveiras Torres-Quiroga, Nan Li

Blood flow promotes emergence of definitive hematopoietic stem cells (HSCs) in the developing embryo, yet the signals generated by hemodynamic forces that influence hematopoietic potential remain poorly defined. Here we show that fluid shear stress endows ...

2015

Antiviral treatment of feline immunodeficiency virus-infected cats with (R)-9-(2-phosphonylmethoxypropyl)-2,6-diaminopurine

Johan Auwerx

Feline immunodeficiency virus (FIV), the causative agent of an acquired immunodeficiency syndrome in cats (feline AIDS), is a ubiquitous health threat to the domestic and feral cat population, also triggering disease in wild animals. No registered antivira ...

Elsevier2015

Dual-regulated Lentiviral Vector for Gene Therapy of X-linked Chronic Granulomatosis

Didier Trono

Regulated transgene expression may improve the safety and efficacy of hematopoietic stem cell (HSC) gene therapy. Clinical trials for X-linked chronic granulomatous disease (X-CGD) employing gammaretroviral vectors were limited by insertional oncogenesis o ...

Elsevier2014

Microbiota-Induced Changes in Drosophila melanogaster Host Gene Expression and Gut Morphology

Bruno Lemaitre, Nichole Broderick

To elucidate mechanisms underlying the complex relationships between a host and its microbiota, we used the genetically tractable model Drosophila melanogaster. Consistent with previous studies, the microbiota was simple in composition and diversity. Howev ...

American Society for Microbiology2014

TLR4- and TRIF-dependent stimulation of B lymphocytes by peptide liposomes enables T cell-independent isotype switch in mice

Pedro Miguel Nunes Pereira de Almeida Reis, Andrea Pfeifer, Marija Vukicevic, Andreas Muhs, Valérie Gafner, Anna Lucia Buccarello

Immunoglobulin class switching from IgM to IgG in response to peptides is generally T cell-dependent and vaccination in T cell-deficient individuals is inefficient. We show that a vaccine consisting of a dense array of peptides on liposomes induced peptide ...

Amer Soc Hematology2013

Highly controlled electrofusion of individually selected cells in dielectrophoretic field cages

Claus Duschl

The prospect of novel therapeutic approaches has renewed the current interest in the fusion of rare cells, like stem cells or primary immune cells. While conventional techniques are only capable of mass fusion, lab-on-a-chip systems often still lack an acc ...

2012

Hyperbranched Polylysine: A Versatile, Biodegradable Transfection Agent for the Production of Recombinant Proteins by Transient Gene Expression and the Transfection of Primary Cells

Harm-Anton Klok, Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Yashas Rajendra, Zuzana Kadlecova

The feasibility of a new transfection agent, HBPL, for the production of recombinant IgG antibody via TGE as well as for the transfection of primary cells is studied. Under the conditions investigated, transfection of CHO-DG44 cells using HBPL results in I ...

2012

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