Concept

Flux (metabolism)

Publications associées (253)

Metabolic control analysis of the central carbon pathway in optimally grown E. coli

Vassily Hatzimanikatis, Ljubisa Miskovic, Keng Cher Soh, Stefano Andreozzi

The engineering of the metabolism requires the building of reliable kinetic models of the metabolic pathways through the integration of the information from heterogeneous data sources. Unfortunately, the quantitative knowledge about fluxes, mechanisms or k ...

2012

Mitochondrial sirtuins and metabolic homeostasis

Johan Auwerx, Eija Riitta Pirinen, Giuseppe Lo Sasso

The maintenance of metabolic homeostasis requires the well-orchestrated network of several pathways of glucose, lipid and amino acid metabolism. Mitochondria integrate these pathways and serve not only as the prime site of cellular energy harvesting but al ...

Elsevier Sci Ltd2012

Cell metabolism monitoring using a microfluidic cartridge with enzyme-based microreactors

Nico de Rooij, Peter Van der Wal, Sara Talaei

We present a novel microfluidic approach for on-line analysis of cell metabolism by simultaneous measurement of two important indicators (glucose and lactate) in a small volume (100 µl) of cell-culture medium. The in-vitro study of cell metabolism is espec ...

2012

A novel approach to find the missing links in genome-scale metabolic models: The BridgeIt mrthod

Vassily Hatzimanikatis, Keng Cher Soh, Marianne Seijo

Genome-scale metabolic reconstructions (GSMRs) are valuable resources in the analysis and understanding of cellular metabolism. They are based on genome sequence and annotation, and they are to develop bottom-up mathematical models of metabolic networks. T ...

2012

Systematic Metabolic Engineering of Recombinant Xylose-Utilizing S. cerevisiae: Strategies for Improving Xylose Uptake Rate

Vassily Hatzimanikatis, Ljubisa Miskovic, Keng Cher Soh

Recent advancements in metabolomics and fluxomics methods and ever-increasing amount of available experimental data necessitate systems-oriented methodologies for efficient and systematic integration of data into consistent large-scale kinetic models capab ...

2012

Mathematical Modeling of Brain Energy Metabolism, Measured with PET and MRS in Rodents

Bernard Lanz

Positron emission tomography (PET) and nuclear magnetic resonance spectroscopy (MRS) are two biomedical measurement techniques developed in the end of the XXth century, which drastically improved the amount of accessible information available in vivo. PET ...

EPFL2012

The Corepressor NCoR1 Antagonizes PGC-1α and Estrogen-Related Receptor α in the Regulation of Skeletal Muscle Function and Oxidative Metabolism

Johan Auwerx

Skeletal muscle exhibits a high plasticity and accordingly can quickly adapt to different physiological and pathological stimuli by changing its phenotype largely through diverse epigenetic mechanisms. The nuclear receptor corepressor 1 (NCoR1) has the abi ...

Amer Soc Microbiology2012

Key electrophysiological, molecular, and metabolic signatures of sleep and wakefulness revealed in primary cortical cultures

Johan Auwerx, Richardus Houtkooper, Cyril Mikhail

Although sleep is defined as a behavioral state, at the cortical level sleep has local and use-dependent features suggesting that it is a property of neuronal assemblies requiring sleep in function of the activation experienced during prior wakefulness. He ...

Soc Neuroscience2012

Systematic characterization of flux and network thermodynamic states for identification of metabolic engineering targets

Vassily Hatzimanikatis, Ljubisa Miskovic, Keng Cher Soh

In order to identify targets for metabolic engineering interventions or understand the physiology of the cell, the first and foremost prerequisite is the knowledge or a hypothesis about the intracellular network state(s). However, even with the advanced me ...

2012

Comparing localized and nonlocalized dynamic 31P magnetic resonance spectroscopy in exercising muscle at 7T

Christine Nabuurs

By improving spatial and anatomical specificity, localized spectroscopy can enhance the power and accuracy of the quantitative analysis of cellular metabolism and bioenergetics. Localized and nonlocalized dynamic 31P magnetic resonance spectroscopy using a ...

Wiley-Blackwell2012

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