Concept

Microscope à statif inversé

Publications associées (60)

SPAD array technology enables fluctuation-contrast super-resolution in a confocal microscope

Edoardo Charbon, Claudio Bruschini, Ivan Michel Antolovic

A fluorescence correlation contrast is a straightforward approach to super-resolution imaging. Combining a SPAD array with a novel detection scheme (ISM), we obtain images with up to x4 times resolution enhancement. ...

OSA-SPIE2021

Correction of spherical surface measurements by confocal microscopy

Toralf Scharf, Wilfried Noell, Jeremy Béguelin

Refractive microlenses are nowadays widely used in optical systems. Characterizing their surface is essential to ensure their quality and to optimize their fabrication process. This is realized by optical surface profilers thanks to their vertical resoluti ...

2020

Quantum correlation measurement with single photon avalanche diode arrays

Edoardo Charbon, Claudio Bruschini, Ivan Michel Antolovic

Temporal photon correlation measurement, instrumental to probing the quantum properties of light, typically requires multiple single photon detectors. Progress in single photon avalanche diode (SPAD) array technology highlights their potential as high-perf ...

2019

Custom Adapter For Extended Field-Of-View Microsphere-Based Scanning Super-Resolution Microscopy

Martinus Gijs, Gergely Huszka

We present a dielectric microsphere-based super resolution optical microscope that can image a sample beyond the classical diffraction limit [1]. The super resolution imaging capability of a single microsphere normally is restricted to an area of similar t ...

IEEE2018

Dielectric-sphere-based microsystem for optical super-resolution imaging

Gergely Huszka

Well-established imaging techniques proved that features below the diffraction limit can be observed optically using so-called super-resolution microscopies, which overcome Abbe's resolution limit. In traditional far-field microscopy, the introduction of f ...

EPFL2018

Dielectric elastomer actuator for the measurement of cell traction forces with sub-cellular resolution

Herbert Shea, Michael James Henry Smith, Samuel Rosset, Alexandre Poulin

We report on the use of dielectric elastomer actuators (DEAs) to measure the traction force field of cells with subcellular resolution. The study of cellular electrochemical and mechanical response to deformation is an important area of research, as mechan ...

Spie-Int Soc Optical Engineering2017

Microsphere-based super-resolution scanning optical microscope

Martinus Gijs, Hui Yang, Gergely Huszka

High-refractive index dielectric microspheres positioned within the field of view of a microscope objective in a dielectric medium can focus the light into a so-called photonic nanojet. A sample placed in such nanojet can be imaged by the objective with su ...

Optical Soc Amer2017

Miniaturized Dielectric Elastomer Actuator for Mechanical Stimulation of Monolayer Cell Cultures

Alexandre Poulin

This thesis advances the field of dielectric elastomer actuators (DEAs) through the development of device designs, fabrication processes, strain characterization technique and modelling tool. It provides the first demonstration that DEAs can be interfaced ...

EPFL2016

Nanoscopy in nonlinear scanning fluorescence imaging systems

Grégoire Pierre Jean Laporte

In the last 30 years, superresolution in optical microscopy has been a major field of research. During this time, different techniques have been created to break the diffraction limit in order to make observations at a nanometric scale. Given that optical ...

EPFL2016

Determination of sub-diffraction-limited organelles and biomolecules using microsphere lenses in combination with fluorescence microscopy

Johan Auwerx, Martinus Gijs, Norman Lionel Moullan, Hui Yang

We propose the use of a high-refractive index glass microsphere in combination with a conventional fluorescence microscope for imaging sub-cellular organelles and biomolecules. The microsphere is placed on a sample that is immersed in water, collects the n ...

Spie-Int Soc Optical Engineering2015

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