Concept
Dénaturation
Publications associées (113)
Development of tools and methods for protein identification: From single molecules to in vivo applications.
As the fundamental machinery orchestrating cellular functions, proteins influence the state of every cell profoundly. As cells exhibit significant variations from one to another, analyzing the proteome on a single-cell level is imperative to unravel their ...
EPFL2024Light-Induced Halide Segregation in 2D and Quasi-2D Mixed-Halide Perovskites
David Lyndon Emsley, Michael Allan Hope, Manuel Cordova, Aditya Mishra
Photoinduced halide segregation hinders widespread application of three-dimensional (3D) mixed-halide perovskites. Much less is known about this phenomenon in lower-dimensional systems. Here, we study photoinduced halide segregation in lower-dimensional mi ...
AMER CHEMICAL SOC2023A fluorescent multi-domain protein reveals the unfolding mechanism of Hsp70
Paolo De Los Rios, Pierre Goloubinoff, Satyam Tiwari, Bruno Claude Daniel Fauvet, Salvatore Assenza
Detailed understanding of the mechanism by which Hsp70 chaperones protect cells against protein aggregation is hampered by the lack of a comprehensive characterization of the aggregates, which are typically heterogeneous. Here we designed a reporter chaper ...
NATURE PORTFOLIO2022Molten globule-like transition state of protein barnase measured with calorimetric force spectroscopy
Understanding how proteins fold into their native structure is a fundamental problem in biophysics, crucial for protein design. It has been hypothesized that the formation of a molten globule intermediate precedes folding to the native conformation of glob ...
NATL ACAD SCIENCES2022Amplitude and phase reconstruction for Low-Energy Electron Holography of individual proteins
Single-molecule imaging methods are of importance in structural biology, and specifically in the imaging of proteins, since they can elucidate conformational variability and structural changes that might be lost in imaging methods relying on averaging proc ...
EPFL2022Competing Effects of Hydration and Cation Complexation in Single-Chain Alginate
Alginic acid, a naturally occurring anionic polyelectrolyte, forms strong physically cross-linked hydrogels in the presence of metal cations. The latter engage in electrostatic interactions that compete with intra- and intermolecular hydrogen bonds, determ ...
2022