Electron tomography

Applied sciences
Génie chimique
Nanotechnologie
Nanotechnologie

Publications associées (27)

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Novel features of centriole polarity and cartwheel stacking revealed by cryo-tomography

Pierre Gönczy, Davide Demurtas, Georgios Hatzopoulos, Sergey Nazarov, Alexandra Bezler, Paul Guichard, Veronika Nemcíková Villímová

Centrioles are polarized microtubule-based organelles that seed the formation of cilia, and which assemble from a cartwheel containing stacked ring oligomers ofSAS-6 proteins. A cryo-tomography map of centrioles from the termite flagellateTrichonymphaspp. ...

2020

Reconstruction Methods for Cryo-Electron Microscopy: From Model-based to Data-driven

Laurène Donati

The topic of this thesis is the development of new reconstruction methods for cryo-electron microscopy (cryo-EM). Cryo-EM has revolutionized the field of structural biology over the last decade and now permits the regular discovery of biostructures. Yet, t ...

EPFL2020

Gold nanoparticles as a new tool in amyloid studies

Urszula Beata Cendrowska

The misfolding and self-assembly of proteins into fibrils is a hallmark of several neurodegenerative and systemic diseases. These disease-associated proteins have the propensity to form fibrils with a cross-Î² sheet structure, called amyloids. Amyloids can ...

EPFL2020

Structure and transformation of bacteriophage A511 baseplate and tail upon infection of Listeria cells

Sergey Nazarov, Petr Leiman, Ricardo Cesar Guerrero Ferreira

Contractile injection systems (bacteriophage tails, type VI secretions system, R-type pyocins, etc.) utilize a rigid tube/contractile sheath assembly for breaching the envelope of bacterial and eukaryotic cells. Among contractile injection systems, bacteri ...

WILEY2019

Eigenmode Analysis in Plasmonics: Application to Second Harmonic Generation and Electron Energy Loss Spectroscopy

Gabriel David Bernasconi

Eigenmodes are central to the study of resonant phenomena in all areas of physics. However, their use in nano-optics seems to have been hindered and delayed for various reasons. First, due to their small size, the response of nanostructures to a far-field ...

EPFL2019

High-Contrast Imaging of Nanodiamonds in Cells by Energy Filtered and Correlative Light-Electron Microscopy: Toward a Quantitative Nanoparticle-Cell Analysis

Theo Lasser

Fluorescent nanodiamonds (fNDs) represent an emerging class of nanomaterials offering great opportunities for ultrahigh resolution imaging, sensing and drug delivery applications. Their biocompatibility, exceptional chemical and consistent photostability r ...

2019

Strain-induced dissolution of Y-Ti-O nano-oxides in a consolidated ferritic oxide dispersion strengthened (ODS) steel

Roland Logé, Mathijs Pieter van der Meer

The present study shows the influence of severe plastic deformation on highly stable Y-Ti-O nano-oxides present in ferritic ODS alloys used for nuclear applications. An innovative strain path implying alternated compressions was used to deform the material ...

2018

Compressed sensing for STEM tomography

Michaël Unser, Masih Nilchian, Laurène Donati

A central challenge in scanning transmission electron microscopy (STEM) is to reduce the electron radiation dosage required for accurate imaging of 3D biological nano-structures. Methods that permit tomographic reconstruction from a reduced number of STEM ...

2017

Compressed Sensing for Dose Reduction in STEM Tomography

Michaël Unser, Laurène Donati, Masih Nilchian

We designed a complete acquisition-reconstruction framework to reduce the radiation dosage in 3D scanning transmission electron microscopy (STEM). Projection measurements are acquired by randomly scanning a subset of pixels at every tilt-view (i.e., random ...

IEEE2017

Ex-situ AlN seed layer for (0001)-textured Al0.84Sc0.16N thin films grown on SiO2 substrates

Paul Muralt, Silviu Cosmin Sandu, Stefan Mertin, Vladimir Pashchenko, Mohammad Fazel Parsapour Kolour

It is more difficult to nucleate AlN-ScN alloy thin films (AlScN) in pure (0001)-texture than it is with pure AlN thin films. AlN thus can serve as seed layer for AlScN. Equipment limitations may lead to the problem of a vacuum break between AlN and AlScN ...

Ieee2017

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High-Contrast Imaging of Nanodiamonds in Cells by Energy Filtered and Correlative Light-Electron Microscopy: Toward a Quantitative Nanoparticle-Cell Analysis

Theo Lasser

2019