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In this letter, we show the results achieved using a hydrogenated amorphous silicon photosensor for a "label-free" deoxyribonucleic acid (DNA) analysis based on the measurements of the absorbance in the ultraviolet range. The optimization of the sensor str ...
During a previous, exploratory project in our group, it was observed that duplexes formed by oligonucleotides modified with polar 2'-O-substituents were more tolerant towards the addition of 1,2-dimethoxyethane (DME) than RNA and DNA duplexes [1]. Inspired ...
Nanopores are under development for the detection of a variety of analytes and the investigation of chemical reactions at the single molecule level. In particular, the analysis of nucleic acid molecules is under intense investigation, including the develop ...
This paper describes a heterogeneous oligonucleotide-hybridization assay based on hot electron-induced electrochemiluminescence (HECL) of a rhodamine label. Thin oxide-film coated aluminum and silicon electrodes were modified with an aminosilane layer and ...
In this paper we will discuss a few basic concepts concerning the use of evanescent optical fields for the excitation of fluorescent chromophores placed near the interface. The observation of enhanced fluorescence from chromophores excited by surface plasm ...
This paper describes a heterogeneous DNA-hybridization assay based on electrochemiluminescence (ECL) detection on gold electrodes. Short, 15-mer oligonucleotides were conjugated with a synthesized electrochemiluminescent label, bis(2,2′-bipyridine)-5-isoth ...
The invention relates to an apparatus for performing chemical assays in an aqueous medium. The apparatus contains a reaction chamber(s) and a liquid in-flow channel connected to each chamber. The flow of liquid through the fluid in-flow channel to the reac ...
TiO2/ITO modified electrodes were developed to quantitatively photooxidize adsorbed ds-DNA and to study the effect of antioxidants as ds-DNA protecting agents. TiO2 films are used for efficient ds-DNA immobilization, for ds- DNA oxidation through photogene ...
Telomerase is the ribonucleoprotein reverse transcriptase that adds telomeric DNA repeats to the ends of chromosomes. This involves annealing of the telomerase RNA template to the 3' end of the chromosome, reverse transcription of the RNA template by the t ...
This work presents a simple microfluidic device with an integrated thin-film heater for studies of DNA hybridization kinetics and double-stranded DNA melting temperature measurements. The heating characteristics of the device were evaluated with a novel, n ...
