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Publications associées (59)

Integrated field, laboratory, and theoretical study of PKD spread in a Swiss prealpine river

Andrea Rinaldo, Enrico Bertuzzo, Lorenzo Mari, Luca Carraro, Marino Gatto

Proliferative kidney disease (PKD) is a major threat to wild and farmed salmonid populations because of its lethal effect at high water temperatures. Its causative agent, the myxozoan Tetracapsuloides bryosalmonae, has a complex lifecycle exploiting freshw ...

National Academy of Sciences2017

Interaction of silver nanoparticles with algae and fish cells: a side by side comparison

Kristin Schirmer, Laura Sigg, Renata Behra, Yang Yue, Xiaomei Li

Background: Silver nanoparticles (AgNP) are widely applied and can, upon use, be released into the aquatic environment. This raises concerns about potential impacts of AgNP on aquatic organisms. We here present a side by side comparison of the interaction ...

Biomed Central Ltd2017

Ultrathin Alumina Membranes as Scaffold for Epithelial Cell Culture from the Intestine of Rainbow Trout

Philippe Renaud, Kristin Schirmer, Songmei Wu, Carolin Drieschner

Permeable membranes are indispensable for in vitro epithelial barrier models. However, currently available polymer-based membranes are low in porosity and relatively thick, resulting in a limited permeability and unrealistic culture conditions. In this stu ...

Amer Chemical Soc2017

An epidemiological model for proliferative kidney disease in salmonid populations

Andrea Rinaldo, Enrico Bertuzzo, Lorenzo Mari, Luca Carraro, Marino Gatto

Background: Proliferative kidney disease (PKD) affects salmonid populations in European and North-American rivers. It is caused by the endoparasitic myxozoan Tetracapsuloides bryosalmonae, which exploits freshwater bryozoans and salmonids as hosts. Inciden ...

Biomed Central Ltd2016

Silver nanoparticles inhibit fish gill cell proliferation in protein-free culture medium

Kristin Schirmer, Laura Sigg, Renata Behra, Yang Yue

While short-term exposures of vertebrate cells, such as from fish, can be performed in defined, serum-free media, long-term cultures generally require addition of growth factors and proteins, normally supplied with a serum supplement. However, proteins are ...

Taylor & Francis Ltd2016

A novel two-compartment barrier model for investigating nanoparticle transport in fish intestinal epithelial cells

Kristin Schirmer, Laura Sigg

We introduce a novel in vitro rainbow trout intestinal barrier model and demonstrate its suitability for investigating nanoparticle transport across the intestinal epithelium. Rainbow trout (Oncorhynchus mykiss) intestinal cells (RTgutGC) were grown as mon ...

Royal Soc Chemistry2016

Silver nanoparticle-protein interactions in intact rainbow trout gill cells

Kristin Schirmer, Laura Sigg, Renata Behra, Yang Yue

Upon contact with biota, nanoparticles can bind to proteins, which coat the nanoparticles and form a nanoparticle-protein corona. Knowledge of corona proteins is therefore important for a mechanistic understanding of how nanoparticles interact with biomole ...

Royal Soc Chemistry2016

Effect of media composition on bioavailability and toxicity of silver and silver nanoparticles in fish intestinal cells (RTgutGC)

Kristin Schirmer

To understand conditions affecting bioavailability and toxicity of citrate-coated silver nanoparticles (cit-AgNP) and dissolved silver at the luminal enterocyte interface, we exposed rainbow trout (Oncorhynchus mykiss) gut cells (RTgutGC) in media of contr ...

Taylor & Francis Ltd2016

An epidemic model for the interactions between thermal regime of rivers and transmission of proliferative kidney disease in salmonid fish

Andrea Rinaldo, Enrico Bertuzzo, Lorenzo Mari, Luca Carraro, Marino Gatto

Proliferative kidney disease (PKD) affects salmonid populations in European and North-American rivers. It is caused by the endoparasitic myxozoan Tetracapsuloides bryosalmonae, which exploits freshwater bryozoans (Fredericella sultana) and salmonids as pri ...

2015

Toxicity of silver nanoparticles to a fish gill cell line: Role of medium composition

Kristin Schirmer, Laura Sigg, Renata Behra, Yang Yue

In aqueous solutions, silver nanoparticle (AgNP) behavior is affected by a variety of factors which lead to altered AgNP size and toxicity. Our research aims to explore the effect of media composition on citrate-coated AgNP (cit-AgNP) behavior and toxicity ...

Informa Healthcare2015

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