Concept

Méthode immuno-enzymatique ELISA

Publications associées (21)

Exploring new therapeutic strategies for Huntington's Disease

Huntington’s disease (HD) is an inherited neurodegenerative disorder caused by an extended poly-glutamine tract in the huntingtin gene (HTT). Despite intensive efforts in understanding its pathogenesis over the decades, effective treatments for HD remain u ...

EPFL2014

Total serum IgE quantification by microfluidic ELISA using magnetic beads

Hubert Girault, Anne-Laure Gassner

The present work reports on the quantification of total IgE in human serum using a microanalytical device whose fluidics is driven by gravity and capillary forces only. Thanks to the eight parallel microchannels in each microchip, calibration and sample an ...

Springer Verlag2012

Automated Analysis of Rare Biomarkers Using a Magnetic Bead Surface Coverage Assay Coupled with Chaotic Mixing on a Microfluidic Chip

Hüseyin Cumhur Tekin

Extremely sensitive protein detection is crucial for early diagnosis and monitoring of diseases. Present day’s limit of detection for an antibody-antigen recognition-based immunoassay in serum without having to use special target antigen amplification tech ...

EPFL2012

Capillary Microfluidic Chips for Point-of-Care Testing

Luc Gervais

Research on microfluidic devices for biological analysis has progressed sufficiently to be developed into point-of-care diagnostics products. The goal of this thesis is to improve multiple aspects of capillary-driven microfluidic devices. In particular, th ...

EPFL2011

Microfluidic Immunoassays Based on Self-Assembled Magnetic Bead Patterns and Time-Resolved Luminescence Detection

Venkataragavalu Sivagnanam

Microfluidic bio-assays have emerged as the most privileged solutions and provide the basis for the realization of miniaturized bio-analytical systems and clinical diagnostic devices that are portable, user-friendly and cost-effective (Lab-on-a-chip). Two ...

EPFL2010

On-chip immunoassay using electrostatic assembly of streptavidin-coated bead micropatterns

Martinus Gijs, Caroline Vandevyver, Venkataragavalu Sivagnanam

We propose an original concept for a sandwich immunoassay that is completely performed on-chip using streptavidin-coated beads as substrate. The latter are electrostatically self-assembled on aminosilane micropatterns at the bottom of a microfluidic channe ...

2009

On-chip immunoassay using electrostatic assembly of streptavidin-coated bead micropatterns

Martinus Gijs, Caroline Vandevyver, Venkataragavalu Sivagnanam, Hui Yang, Bo Song

2009

On-chip antibody handling and colorimetric detection in a magnetic droplet manipulation system

Martinus Gijs, Ulrike Lehmann, Damien de Courten, Virendra Kumar Parashar, Caroline Vandevyver

Miniaturized system for bioanalysis have been constantly gaining importance and interest within the recent years. The reduction of sample consumption and reaction time are some of the major advantages of such lab-on-a-chip applications. One of the greatest ...

2007

The affinity precipitation for the isolation of biomolecules

Product isolation increasingly becomes a major bottleneck in molecular biotechnology. This is especially the case for the sensitive proteineous substances (recombinant proteins, antibodies) and lately also DNA, usually produced in low concentration. The cu ...

EPFL2007

Engineering a thermostable human prolyl endopeptidase for antibody-directed enzyme prodrug therapy

Christian Heinis, Dario Neri

We present a new antibody-directed enzyme prodrug therapy strategy (ADEPT) based on a post-proline cleaving endopeptidase and prodrugs, in which cytotoxic moieties are linked to a proline-contg. peptide. Human prolyl endopeptidase was expressed in Escheric ...

2004

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