Concept
Biolistique
Publications associées (87)
Core-shell Structured Chitosan-polyethylenimine Nanoparticles for Gene Delivery: Improved Stability, Cellular Uptake, and Transfection Efficiency
Sandrine Gerber, Perrine Agnes Edith Robin, Laura Camille Louise Nicolle
Gene therapy emerged as a promising treatment option for acquired and inherited diseases. The delivery of nucleic acids relies on vectors that condense and encapsulate their cargo. Especially non-viral gene delivery systems are of increasing interest. Howe ...
2022Engineering synthetic adhesins for biophysical investigation and rewiring of host-microbe interactions
Host attachment is often a critical step in the onset of pathogenesis. To attach to host cells, bacteria have evolved a range of adhesins that bind to specific receptors. Some of these adhesins have been thoroughly characterized using biochemical technique ...
EPFL2022Biotechnology Applications of Nanocarbons in Plant and Algal Systems
Ardemis Anoush Boghossian, Alice Judith Gillen, Alessandra Antonucci
The unique combination of structural, optical, and electronic properties of nanocarbons (NCs) has made these materials particularly attractive for various applications in biotechnology and biomedicine, ranging from sensing, gene-delivery, bioimaging and th ...
Royal Society of Chemistry2021Efficient viral transduction in mouse inner ear hair cells with utricle injection and AAV9-PHP.B
Bernard Schneider, Paola Andrea Solanes Vega
Viral delivery of exogenous coding sequences into the inner ear has the potential for therapeutic benefit for patients suffering genetic or acquired hearing loss. To devise improved strategies for viral delivery, we investigated two injection techniques, r ...
2020A ligand-based system for receptor-specific delivery of proteins
Gene delivery using vector or viral-based methods is often limited by technical and safety barriers. A promising alternative that circumvents these shortcomings is the direct delivery of proteins into cells. Here we introduce a non-viral, ligand-mediated p ...
Springer2019Recombinant protein production from stable mammalian cell lines and pools
David Hacker, Sowmya Balasubramanian
We highlight recent developments for the production of recombinant proteins from suspension-adapted mammalian cell lines. We discuss the generation of stable cell lines using transposons and lentivirus vectors (non-targeted transgene integration) and site- ...
Elsevier2016Multigene expression in stable CHO cell pools generated with the piggyBac transposon system
Florian Maria Wurm, David Hacker, Sowmya Balasubramanian
Heterogenous populations of recombinant cells (cell pools) stably expressing 1-4 transgenes were generated from Chinese hamster overy (CHO) cells with the piggyBac (PB) transposon system. The cell pools produced different combinations of three model protei ...
American Chemical Society2016Rapid recombinant protein production from piggyBac transposon-mediated stable CHO cell pools
Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Zuzana Kadlecova, Sowmya Balasubramanian
Heterogeneous populations of stably transfected cells (cell pools) can serve for the rapid production of moderate amounts of recombinant proteins. Here, we propose the use of the piggyBac (PB) transposon system to improve the productivity and long-term sta ...
Elsevier2015Study of transposon-mediated cell pool and cell line generation in CHO cells
The goal of this thesis was to evaluate the use of artificial transposon systems for the generation of recombinant cell pools and cell lines with Chinese hamster ovary (CHO) cells as the production host. Transposons are naturally occurring genetic elements ...
EPFL2015