Concept
Peroxydase de raifort
Publications associées (7)
Combining fluorescence-based image segmentation and automated microfluidics for ultrafast cell-by-cell assessment of biomarkers for HER2-type breast carcinoma
Martinus Gijs, Daniel Migliozzi, Huu Tuan Nguyen
Immunohistochemistry (IHC) is one of the main clinical techniques for biomarker assessment on tissue biopsies. It consists in chromogenic labeling with specific antibodies, followed by optical imaging, and it is used for diagnosis and therapeutic targeting ...
SPIE-Intl Soc Optical Eng2019Detection of mRNA by Whole Mount in situ Hybridization and DNA Extraction for Genotyping of Zebrafish Embryos
Andrew Charles Oates, Rachna Narayanan
In situ hybridization is used to visualize the spatial distribution of gene transcripts in tissues and in embryos, providing important information about disease and development. Current methods involve the use of complementary riboprobes incorporating non- ...
BIO-PROTOCOL2019Single Cell Electrochemiluminescence Imaging: From the Proof-of-Concept to Disposable Device-Based Analysis
Andreas Stephan Lesch, Milica Jovic
We report here the development of coreactant-based electro-generated chemiluminescence (ECL) as a surface-confined microscopy to image single cells and their membrane proteins. Labeling the entire cell membrane allows one to demonstrate that, by contrast w ...
American Chemical Society2017Continuous quantification of HER2 expression by microfluidic precision immunofluorescence estimates HER2 gene amplification in breast cancer
Martinus Gijs, Diego Gabriel Dupouy, Ata Tuna Ciftlik, Maryse Fiche, Déborah Heintze
Chromogenic immunohistochemistry (IHC) is omnipresent in cancer diagnosis, but has also been criticized for its technical limit in quantifying the level of protein expression on tissue sections, thus potentially masking clinically relevant data. Shifting f ...
Nature Publishing Group2016A selective chemiluminescence detection method for reactive oxygen species involved in oxygen reduction reaction on electrocatalytic materials
Christos Comninellis, Pauline Bornoz
An original selective and quantitative detection method for direct access to reactive oxygen species (ROS) generated in near-neutral aqueous solutions during transient electrochemical experiments is described herein. Several chemiluminescent probes, which ...
Pergamon-Elsevier Science Ltd2013A wireless electrochemiluminescence detector applied to direct and indirect detection for electrophoresis on a microfabricated glass device
A novel electrochemiluminescence (ECL) detector is presented in this article. The detector is applied for micellar electrokinetic chromatographic separation of dichlorotris(2,2′-bipyridyl)ruthenium(II) hydrate [Ru(bpy)] and dichlorotris(1,10-phenanthroline ...
2001Directed molecular evolution of cytochrome c peroxidase
Kai Johnsson, Petra Tafelmeyer
Cytochrome c peroxidase (CCP) from Saccharomyces cerevisiae was subjected to directed mol. evolution to generate mutants with increased activity against the classical peroxidase substrate, guaiacol, thus changing the substrate specificity of CCP from the p ...
2000