Concept

Cytolyse

Publications associées (19)

Portable automated osmolality and pH adjusting apparatus for pretreatment of environmental water samples delivered into a cell-based biosensor

Nico de Rooij, Peter Van der Wal, Sara Talaei

Using our portable in-line fluidic pretreatment apparatus we have adjusted the osmolality and pH of Swiss river samples automatically without any dilution or degradation of the samples. The samples will be tested by means of a cell-based water quality bios ...

2012

Mechanism of photocatalytic bacterial inactivation on TiO2 films involving cell-wall damage and lysis

César Pulgarin, Juan Kiwi

This article addresses the cell wall damage of Escherichia coil (from now on E. coil) by TiO2 suspensions. The dynamics of TiO2 photocatalysis by thin films layers is described. The films were characterized by FTIR spectroscopy and atomic force microscopy ...

Elsevier2012

Single-Cell Analysis of Mycobacterial Persistence Using Microfabricated Tools

Meltem Elitas

Bacterial cells behave as individuals despite being genetically identical and subject to the same environment. Although the underlying mechanisms of cellular individuality are not well understood, temporal variation of gene expression and protein levels in ...

EPFL2012

Formation of assimilable organic carbon during oxidation of natural waters with ozone, chlorine dioxide, chlorine, permanganate, and ferrate

Urs von Gunten

Five oxidants, ozone, chlorine dioxide, chlorine, permanganate, and ferrate were studied with regard to the formation of assimilable organic carbon (AOC) and oxalate in absence and presence of cyanobacteria in lake water matrices. Ozone and ferrate formed ...

2011

Cell sorting by dielectrophoresis for evaluation of lysis efficiency in continuous flow

Philippe Renaud, Niccolo Piacentini

This paper reports on a device capable of electrical cell lysis and evaluation of lysis efficiency in continuous flow using dielectrophoretic cell sorting. The device uses an innovative design, called liquid electrodes, to perform both cell lysis and cell ...

2010

Multiple-frequency Impedance Measurements in Continuous Flow for the Evaluation of Electrical Lysis of Yeast Cells

Philippe Renaud, Niccolo Piacentini, Willyan Hasenkamp Carreira

his paper reports on an electrical lysis device with integrated cell counting and evaluation of the lysis efficiency by multiplefrequency impedance measurements. Conventional techniques use chemical reagents to lyse cells, causing unwanted interference wit ...

Elsevier2010

Dynamics of E. coli membrane cell peroxidation during TiO2 photocatalysis studies by ATR-FTIR spectroscopy and AFM microscopy

Juan Kiwi

Escherichia coli (E. coli) photokilling due to the TiO2 under light irradiation in a batch reactor was studied by using of attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) and atomic force microscopy (AFM). The ATR-FTIR spectr ...

2005

Not as simple as just punching a hole

Françoise Gisou van der Goot Grunberg, Laurence Gouzi Abrami

Like a variety of other pathogenic bacteria, Aeromonas hydrophila secretes a pore-forming toxin that contribute to its virulence. The last decade has not only increased our knowledge about the structure of this toxin, called aerolysin, but has also shed li ...

2001

An unmodified form of the ColE2 lysis protein, an envelope lipoprotein, retains reduced ability to promote colicin E2 release and lysis of producing cells

Stewart Cole

Site-directed mutagenesis was used to replace the codon for the N-terminal cysteine residue of pColE2-P9-encoded mature lysis protein (CelB) by an arginine codon. In contrast to the wild-type CelB protein, the product of the mutated gene, which has an alte ...

1987

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