Publications de Philippe Pierre Girard

Generation of recombinant Chinese hamster ovary cell lines by microinjection

Florian Maria Wurm, Martin Jordan, Madiha Derouazi, Philippe Pierre Girard

Microinjection is a gene transfer technique enabling partial control of plasmid delivery into the nucleus or cytoplasm of cultured animal cells. Here this method was used to establish various recombinant mammalian cell lines. The injection volume was estim ...

Kluwer Academic Publishers, Springer2006

Orbital shaker technology for the cultivation of mammalian cells in suspension

Florian Maria Wurm, David Hacker, Martin Jordan, Natalie Muller, Philippe Pierre Girard

For large-scale applications in biotechnology, cultivation of mammalian cells in suspension is an essential prerequisite. Typically, suspension cultures are grown in glass spinner flasks filled to less than 50% of the nominal volume. We propose a superior ...

2005

Transient gene expression in suspension HEK-293 cells: application to large-scale protein production

Florian Maria Wurm, Lucia Baldi Unser, Natalie Muller, Philippe Pierre Girard

Recent advances in genomics, proteomics, and structural biology raised the general need for significant amounts of pure recombinant protein (r-protein). Because of the difficulty in obtaining in some cases proper protein folding in bacteria, several method ...

2005

Transient gene expression in suspension HEK-293 cells: application to large-scale protein production

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Philippe Pierre Girard

Springer2003

Transient gene expression: A novel mammalian cell-based technology for recombinant protein production. A historical and technical perspective

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Martin Jordan, Natalie Muller, Philippe Pierre Girard, Frédéric Grosjean, Jason Wright

Springer2002

Rapid protein expression in mammalian cells : large-scale transient transfection

Philippe Pierre Girard

EPFL2002