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Charles Roduit

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Publications associées (11)

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A universal fluid cell for the imaging of biological specimens in the atomic force microscope

Giovanni Dietler, Sandor Kasas, Giovanni Longo, Charles Roduit, Livan Bladimir Alonso Sarduy, Bhaskar Saha

Recently, atomic force microscope (AFM) manufacturers have begun producing instruments specifically designed to image biological specimens. In most instances, they are integrated with an inverted optical microscope, which permits concurrent optical and AFM ...

Wiley-Blackwell2013

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The determination of the characteristics of micro-organisms in clinical specimens is essential for the rapid diagnosis and treatment of infections. A thorough investigation of the nanoscale properties of bacteria can prove to be a fundamental tool. Indeed, ...

Wiley-Blackwell2012

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Stiffness tomography is a new atomic force microscopy imaging technique that allows highlighting structures located underneath the surface of the sample. In this imaging mode, such structures are identified by investigating their mechanical properties. We ...

Wiley-Blackwell2012

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The antigen-presenting cell-expressed CD40 is implied in the regulation of counteractive immune responses such as induction of pro-inflammatory and anti-inflammatory cytokines interleukin (IL)-12 and IL-10, respectively. The mechanism of this duality in CD ...

Elsevier2012

Atomic Force Microscopy Stiffness Tomography on Living Arabidopsis thaliana Cells Reveals the Mechanical Properties of Surface and Deep Cell-Wall Layers during Growth

Giovanni Dietler, Sandor Kasas, Charles Roduit, Christian Fankhauser

Cell-wall mechanical properties play a key role in the growth and the protection of plants. However, little is known about genuine wall mechanical properties and their growth-related dynamics at subcellular resolution and in living cells. Here, we used ato ...

Elsevier2012

Septins are conserved GTPases that form filaments and are required for cell division. During interphase, septin filaments associate with cellular membrane and cytoskeleton networks, yet the functional significance of these associations have, to our knowled ...

Elsevier2011

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AFM; DNA topology; Human topoisomerase II; Binding geometry; Time-lapse imaging; Protein–DNA interaction ...

Elsevier2011

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The atomic force microscope is a convenient tool to probe living samples at the nanometric scale. Among its numerous capabilities, the instrument can be operated as a namo-indenter to gather information about the mechanical properties of the sample. In thi ...

2009

The aim of this thesis was to explore the cell mechanical properties using the Atomic Force Microscope (AFM). The cell membrane contains lipids microdomains, called rafts, enriched in cholesterol and sphingolipids. The rafts are believed to play an importa ...

EPFL2008

Elastic membrane heterogeneity of living cells revealed by stiff nanoscale membrane domains

Giovanni Dietler, Françoise Gisou van der Goot Grunberg, Paolo De Los Rios, Sandor Kasas, Charles Roduit, Pascal Steiner, Alexandre Yersin

Many approaches have been developed to characterize the heterogeneity of membranes in living cells. In this study, the elastic properties of specific membrane domains in living cells are characterized by atomic force microscopy. Our data reveal the existen ...

Elsevier2008