Publications de Jovan Simicevic

Transcription factor proteomics - Tools, applications, and challenges

Transcription factors (TFs) are a family of DNA-binding proteins whose gene regulatory capabilities are of vital importance in defining the molecular state of a cell. Despite their biological significance, our understanding of TF behavior and function is s ...

Wiley-Blackwell2017

A mammalian transcription factor-specific peptide repository for targeted proteomics

Bart Deplancke, Jovan Simicevic, Romain Hamelin, Marc Moniatte

Site-specific transcription factors (TFs) play an essential role in mammalian development and function as they are vital for the majority of cellular processes. Despite their biological importance, TF proteomic data is scarce in the literature, likely due ...

Wiley-Blackwell2015

A yeast one-hybrid and microfluidics-based pipeline to map mammalian gene regulatory networks

Bart Deplancke, Alina Isakova, Sebastian Martin Waszak, Jovan Simicevic, Sunil Kumar Raghav, Antonina Iagovitina, Carine Delattre-Gubelmann, Jean-Daniel Feuz

The comprehensive mapping of gene promoters and enhancers has significantly improved our understanding of how the mammalian regulatory genome is organized. An important challenge is to elucidate how these regulatory elements contribute to gene expression b ...

Nature / European Molecular Biology Organization2013

Dissecting Gene Regulatory Networks Using Targeted Quantitative Proteomics

Jovan Simicevic

Gene regulatory networks control gene expression levels, and therefore play an essential role in mammalian development and function. Regulation of gene expression is the result of a complex interplay between DNA regulatory elements and their binding partne ...

EPFL2012

DNA-centered approaches to characterize regulatory protein–DNA interaction complexes

Bart Deplancke, Jovan Simicevic

Gene regulation is mediated by site-specific DNA-binding proteins or transcription factors (TFs), which form protein complexes at regulatory loci either to activate or repress the expression of a target gene. The study of the dynamic properties of these re ...

Royal Society of Chemistry2010