Publications de Andrzej Kulik | EPFL Graph Search

Infrared nanospectroscopic mapping of a single metaphase chromosome

Giovanni Dietler, Andrzej Kulik, Francesco Simone Ruggeri, Ewelina Wioletta Lipiec

The integrity of the chromatin structure is essential to every process occurring within eukaryotic nuclei. However, there are no reliable tools to decipher the molecular composition of metaphase chromosomes. Here, we have applied infrared nanospectroscopy ...

Oxford University Press (OUP)2019

Nanomechanics of multidomain neuronal cell adhesion protein contactin revealed by single molecule AFM and SMD (vol 7, 2017)

Giovanni Dietler, Andrzej Kulik, Karolina Anna Mikulska Ruminska

Nature Research2018

Nanoscale Investigation into the Cellular Response of Glioblastoma Cells Exposed to Protons

Giovanni Dietler, Andrzej Kulik, Ewelina Wioletta Lipiec

2018

Nanomechanics of multidomain neuronal cell adhesion protein contactin revealed by single molecule AFM and SMD

Giovanni Dietler, Andrzej Kulik, Karolina Anna Mikulska Ruminska

Contactin-4 (CNTN4) is a complex cell adhesion molecule (CAM) localized at neuronal membranes, playing a key role in maintaining the mechanical integrity and signaling properties of the synapse. CNTN4 consists of six immunoglobulin C2 type (IgC2) domains a ...

Springer Nature2017

MechStiff: A New Tool for Evaluating Stress-Induced Dynamics and Application to Cell Adhesion Proteins

Giovanni Dietler, Andrzej Kulik, Karolina Anna Mikulska Ruminska

Cell Press2017

Can Dissipative Properties of Single Molecules Be Extracted from a Force Spectroscopy Experiment?

Giovanni Dietler, Andrzej Kulik, Serguei Sekatski, Dmitry Klinov, Fabrizio Benedetti

We performed dynamic force spectroscopy of single dextran and titin 127 molecules using small-amplitude and low-frequency (40-240 Hz) dithering of an atomic force microscope tip excited by a sine wave voltage fed onto the tip-carrying piezo. We show that f ...

Elsevier2016

Nanoscale resolution in infrared imaging of protein-containing lipid membranes

Giovanni Dietler, Andrzej Kulik

The precise imaging of biomolecular entities contributes to an understanding of the relationship between their structure and function. However, the resolution of conventional infrared microscopic imaging is diffraction limited and does not exceed a few mic ...

Royal Society of Chemistry2015

Probing fibronectin-antibody interactions using AFM force spectroscopy and lateral force microscopy

Andrzej Kulik, Kyumin Lee

The first experiment showing the effects of specific interaction forces using lateral force microscopy (LFM) was demonstrated for lectin-carbohydrate interactions some years ago. Such measurements are possible under the assumption that specific forces stro ...

Beilstein-Institut2015

Nanoscale spatially resolved infrared spectra from single microdroplets

Giovanni Dietler, Andrzej Kulik, Francesco Simone Ruggeri

Droplet microfluidics has emerged as a powerful platform allowing a large number of individual reactions to be carried out in spatially distinct microcompartments. Due to their small size, however, the spectroscopic characterisation of species encapsulated ...

Royal Society of Chemistry2014

Assessing dystrophies and other muscle diseases at the nanometer scale by atomic force microscopy

Giovanni Dietler, Harald Brune, Andrzej Kulik, Nicolas Mermod, Guillaume Witz, Stefania Puttini

Aim: Atomic force microscopy nanoindentation of myofibers was used to assess and quantitatively diagnose muscular dystrophies from human patients. Materials & methods: Myofibers were probed from fresh or frozen muscle biopsies from human dystrophic patient ...

Future Medicine2014