Publications de Andrzej Kulik | EPFL Graph Search

Infrared nanospectroscopic mapping of a single metaphase chromosome

Giovanni Dietler, Andrzej Kulik, Francesco Simone Ruggeri, Ewelina Wioletta Lipiec

The integrity of the chromatin structure is essential to every process occurring within eukaryotic nuclei. However, there are no reliable tools to decipher the molecular composition of metaphase chromosomes. Here, we have applied infrared nanospectroscopy ...

Oxford University Press (OUP)2019

Nanomechanics of multidomain neuronal cell adhesion protein contactin revealed by single molecule AFM and SMD

Giovanni Dietler, Andrzej Kulik, Karolina Anna Mikulska Ruminska

Contactin-4 (CNTN4) is a complex cell adhesion molecule (CAM) localized at neuronal membranes, playing a key role in maintaining the mechanical integrity and signaling properties of the synapse. CNTN4 consists of six immunoglobulin C2 type (IgC2) domains a ...

Springer Nature2017

Can Dissipative Properties of Single Molecules Be Extracted from a Force Spectroscopy Experiment?

Giovanni Dietler, Andrzej Kulik, Fabrizio Benedetti, Dmitry Klinov

We performed dynamic force spectroscopy of single dextran and titin 127 molecules using small-amplitude and low-frequency (40-240 Hz) dithering of an atomic force microscope tip excited by a sine wave voltage fed onto the tip-carrying piezo. We show that f ...

Elsevier2016

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The first experiment showing the effects of specific interaction forces using lateral force microscopy (LFM) was demonstrated for lectin-carbohydrate interactions some years ago. Such measurements are possible under the assumption that specific forces stro ...

Beilstein-Institut2015

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The precise imaging of biomolecular entities contributes to an understanding of the relationship between their structure and function. However, the resolution of conventional infrared microscopic imaging is diffraction limited and does not exceed a few mic ...

Royal Society of Chemistry2015

Assessing dystrophies and other muscle diseases at the nanometer scale by atomic force microscopy

Giovanni Dietler, Harald Brune, Andrzej Kulik, Nicolas Mermod, Guillaume Witz, Stefania Puttini

Aim: Atomic force microscopy nanoindentation of myofibers was used to assess and quantitatively diagnose muscular dystrophies from human patients. Materials & methods: Myofibers were probed from fresh or frozen muscle biopsies from human dystrophic patient ...

Future Medicine2014

Nanoscale spatially resolved infrared spectra from single microdroplets

Giovanni Dietler, Andrzej Kulik, Francesco Simone Ruggeri

Droplet microfluidics has emerged as a powerful platform allowing a large number of individual reactions to be carried out in spatially distinct microcompartments. Due to their small size, however, the spectroscopic characterisation of species encapsulated ...

Royal Society of Chemistry2014

Innovative transparent non-scattering TiO2 bactericide thin films inducing increased E. coli cell wall fluidity

César Pulgarin, Juan Kiwi, Andrzej Kulik, Sami Rtimi, Rosendo Sanjines, Mariusz Andrzejczuk

This study presents the first account for transparent, non-scattering TiO2 films on radio-frequency (RF) plasma and ultraviolet light in the C-spectral region (UVC) pretreated polyethylene (PE). These pretreatments allow the PE to bind higher amounts of Ti ...

Elsevier Science Sa2014

MAR-mediated integration of plasmid vectors for in vivo gene transfer and regulation

Andrzej Kulik, Nicolas Mermod, Stefania Puttini, Déborah Ley, Damien Saugy

Background: The in vivo transfer of naked plasmid DNA into organs such as muscles is commonly used to assess the expression of prophylactic or therapeutic genes in animal disease models. Results: In this study, we devised vectors allowing a tight regulatio ...

BioMed Central2013

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In this study, we analyzed multibilayer lipid-protein membranes composed of the photosynthetic light-harvesting complex II (LHCII; isolated from spinach [Spinacia oleracea]) and the plant lipids monogalcatosyldiacylglycerol and digalactosyldiacylglycerol. ...

Amer Soc Plant Biologists2013

Andrzej Kulik

Infrared nanospectroscopic mapping of a single metaphase chromosome

Nanomechanics of multidomain neuronal cell adhesion protein contactin revealed by single molecule AFM and SMD

Can Dissipative Properties of Single Molecules Be Extracted from a Force Spectroscopy Experiment?

Assessing dystrophies and other muscle diseases at the nanometer scale by atomic force microscopy

Nanoscale spatially resolved infrared spectra from single microdroplets

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Infrared nanospectroscopic mapping of a single metaphase chromosome

Nanomechanics of multidomain neuronal cell adhesion protein contactin revealed by single molecule AFM and SMD

Can Dissipative Properties of Single Molecules Be Extracted from a Force Spectroscopy Experiment?

Assessing dystrophies and other muscle diseases at the nanometer scale by atomic force microscopy

Nanoscale spatially resolved infrared spectra from single microdroplets

Innovative transparent non-scattering TiO2 bactericide thin films inducing increased E. coli cell wall fluidity

MAR-mediated integration of plasmid vectors for in vivo gene transfer and regulation

MAR-mediated integration of plasmid vectors for in vivo gene transfer and regulation

Innovative transparent non-scattering TiO2 bactericide thin films inducing increased E. coli cell wall fluidity