A recombinant avidin-producing Mut(+) Pichia pastoris strain was used as a model organism to study the influence of the methanol feeding strategy on the specific product productivity (q(p)) and protein glycosylation. Fed-batch cultivations performed at var ...
The objective of this thesis was to characterize the growth stoichiometry, the specific recombinant protein productivity and the regulation of the alcohol oxidase (AOX) enzyme of a Pichia pastoris Mut+ strain, performing conventional chemostat cultures and ...
