Publications de Florian Charrière

Method and apparatus to measure and compute the amplitude point spread function and associated parameters of a coherent optical imaging system

Christian Depeursinge, Tristan Colomb, Pierre Marquet, Florian Charrière, Mihaela Marian

The invention concerns a method of and an apparatus for the measurement of the Amplitude Point Spread Function (APSF), of an Optical Imaging System (OIS), wherein the APSF is defined as the complex field distribution of the beam delivered by the OIS and or ...

2008

Simultaneous cell morphometry and refractive index measurement with dual- wavelength digital holographic microscopy and dye-enhanced dispersion of perfusion medium

Christian Depeursinge, Pierre Magistretti, Benjamin Rappaz, Florian Charrière, Pierre Marquet

Digital holographic microscopy (DHM) allows optical-path-difference (OPD) measurements with nanometric accuracy. OPD induced by transparent cells depends on both the refractive index (RI) of cells and their morphology. This Letter presents a dual-wavelengt ...

2008

Axial sub-nanometer accuracy in digital holographic microscopy

Christian Depeursinge, Tristan Colomb, Etienne Cuche, Frédéric Montfort, Pierre Marquet, Florian Charrière, Jonas Kühn

2008

On the complex three-dimensional amplitude point spread function of lenses and microscope objectives: theoretical aspects, simulations and measurements by digital holography

Christian Depeursinge, Tristan Colomb, Frédéric Montfort, Pierre Marquet, Florian Charrière, Jonas Kühn, Mihaela Marian

The point spread function is widely used to characterize the three-dimensional imaging capabilities of an optical system. Usually, attention is paid only to the intensity point spread function, whereas the phase point spread function is most often neglecte ...

2007

Real-time dual-wavelength digital holographic microscopy with a single hologram acquisition

Christian Depeursinge, Tristan Colomb, Etienne Cuche, Frédéric Montfort, Pierre Marquet, Florian Charrière, Jonas Kühn

A technique to perform two-wavelengths digital holographic microscopy (DHM) measurements with a single hologram acquisition is presented. The vertical measurement range without phase ambiguity is extended to the micron-range, thanks to the resulting synthe ...

2007

Amplitude point spread function measurement of high NA microscope objectives by digital holographic microscopy

Christian Depeursinge, Tristan Colomb, Pierre Marquet, Florian Charrière, Mihaela Marian

We present here a three-dimensional evaluation of the amplitude point-spread function (APSF) of a microscope objective (MO), based on a single holographic acquisition of its pupil wavefront. The aberration function is extracted from this pupil measurements ...

2007

Influence of shot noise on phase measurement accuracy in digital holographic microscopy

Christian Depeursinge, Tristan Colomb, Benjamin Rappaz, Pierre Marquet, Florian Charrière, Jonas Kühn

Digital Holographic Microscopy (DHM) is a single shot interferometric technique, which provides quantitative phase images with subwavelength axial accuracy. A short hologram acquisition time (down to microseconds), allows DHM to offer a reduced sensitivity ...

2007

Digital holographic microscopy applied to life sciences

Christian Depeursinge, Tristan Colomb, Benjamin Rappaz, Pierre Marquet, Florian Charrière, Jonas Kühn

Digital holographic microscopy (DHM) is applied to life sciences applications and demonstrate its capability of real-time imaging and quantitative measurements of physiological parameters such as cell volume or mean cell hemoglobin concentration (MCHC) of ...

IEEE2007

Shot noise, refractive index tomography and aberrations estimation in digital holographic microscopy

Florian Charrière

The present thesis develops some specific aspects of digital holographic microscopy (DHM), namely the effect of shot noise on the phase image accuracy, the use of DHM in micro-tomography and in aberrations evaluation of a microscope objective (MO). DHM is ...

EPFL2007

WAVE FRONT SENSING METHOD AND APPARATUS

Christian Depeursinge, Tristan Colomb, Etienne Cuche, Frédéric Montfort, Nicolas Aspert, Florian Charrière, Pierre Marquet, Stéphane Bourquin, Mihaela Marian

A new way of mixing instrumental and digital means is described for the general field of wave front sensing. The present invention describes the use, the definition and the utility of digital operators, called digital wave front operators (DWFO) or ... A n ...

2006