Personne
Christof Holliger
Publications associées (207)
Microbial genome collection of aerobic granular sludge cultivated in sequencing batch reactors using different carbon source mixtures
Christof Holliger, Aline Sondra Adler, Laetitia Janine Andrée Cardona, Jaspreet Singh Saini, Pilar Natalia Rodilla Ramírez, Ruizhe Pei
Aerobic granular sludge (AGS) consists of a microbial consortium that has an important role in wastewater treatment. This study investigates AGS microorganisms cultivated in a laboratory-scale sequencing batch reactor. Metagenomic sequencing was conducted ...
Amer Soc Microbiology2024Proteome adaptations of the organohalide-respiring Desulfitobacterium hafniense strain DCB-2 to various energy metabolisms
Christof Holliger, Julien Maillard, Romain Hamelin, Mathilde Stéphanie Willemin, Florence Armand
IntroductionDesulfitobacterium hafniense was isolated for its ability to use organohalogens as terminal electron acceptors via organohalide respiration (OHR). In contrast to obligate OHR bacteria, Desulfitobacterium spp. show a highly versatile energy meta ...
FRONTIERS MEDIA SA2023Sulfurospirillum diekertiae sp. nov., a tetrachloroethene-respiring bacterium isolated from contaminated soil
Christof Holliger, Julien Maillard, Junxiong Wang
Two anaerobic, tetrachloroethene- (PCE-) respiring bacterial isolates, designated strain ACSDCE T and strain ACSTCE, were characterized using a polyphasic approach. Cells were Gram-stain-negative, motile, non-spore-forming and shared a vibrioid- to spirill ...
2023Structure of a membrane-bound menaquinol:organohalide oxidoreductase
Christof Holliger, Henning Paul-Julius Stahlberg, Julien Maillard, Dongchun Ni, Lorenzo Cimmino
Organohalide-respiring bacteria are key organisms for the bioremediation of soils and aquifers contaminated with halogenated organic compounds. The major players in this process are respiratory reductive dehalogenases, corrinoid enzymes that use organohali ...
2023Environment rather than breed or body site shapes the skin bacterial community of healthy sheep as revealed by metabarcoding
Christof Holliger, Alexander Mathis, Emmanuelle Rohrbach, Laetitia Janine Andrée Cardona
Background: The skin is inhabited by a variety of micro-organisms, with bacteria representing the predominant taxon of the skin microbiome. In sheep, the skin bacterial community of healthy animals has been addressed in few studies, only with culture-based ...
2023Disentangle genus microdiversity within a complex microbial community by using a multi-distance long-read binning method: example of Candidatus Accumulibacter
Christof Holliger, Julien Maillard, Aline Sondra Adler, Marco Pagni, Simon Marius Jean Poirier
Complete genomes can be recovered from metagenomes by assembling and binning DNA sequences into metagenome assembled genomes (MAGs). Yet, the presence of microdiversity can hamper the assembly and binning processes, possibly yielding chimeric, highly fragm ...
WILEY2022Assessing the value of kinetic results from biochemical methane potential tests: Reproducibility from a large inter-laboratory study
Kinetic information extracted from biochemical methane potential (BMP) tests is often reported but its value is unclear. Inter-laboratory reproducibility provides a useful indication of its value. Here we extracted estimates of the first-order rate constan ...
2022Organohalide respiration: breakthrough towards the elucidation of the electron-accepting moiety of the respiratory chain
Christof Holliger, Julien Maillard, Adrian Schmid, Lorenzo Cimmino
Background Organohalide respiration (OHR) is a bacterial anaerobic process in which chlorinated compound, e.g. tetrachloroethene (PCE), is used as terminal electron acceptors. Dehalobacter restrictus PER-K23, a paradigmatic organohalide-respiring bacterium ...
2021Organohalide respiration: variations on a common theme
Christof Holliger, Julien Maillard, Mathilde Stéphanie Willemin, Lorenzo Cimmino
Organohalide respiration (OHR) is a bacterial anaerobic respiratory metabolism that makes use of halogenated organic compounds as terminal electron acceptors. While organohalogens have been initially thought to be mainly from anthropogenic origin, thousand ...
2021Conditional essentiality of a complex I-like enzyme in strict anaerobes: the case of Desulfitobacterium
Christof Holliger, Julien Maillard, Romain Hamelin, Mathilde Stéphanie Willemin, Florence Armand
Respiratory complex I is a well-known player in aerobic respiration; it couples the transfer of two electrons from NADH to ubiquinone alongside with proton pumping. Bacterial complex I is usually composed of fourteen subunits forming three different module ...
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