Publications de Giovanni Dietler

An Optical Fiber-Based Nanomotion Sensor for Rapid Antibiotic and Antifungal Susceptibility Tests

Giovanni Dietler, Sandor Kasas, María Inés Villalba, Serguei Sekatski, Anne-Céline Verenna Kohler, Jiangtao Zhou, Leonardo Venturelli

The emergence of antibiotic and antifungal resistant microorganisms represents nowadays a major public health issue that might push humanity into a post-antibiotic/antifungal era. One of the approaches to avoid such a catastrophe is to advance rapid antibi ...

Amer Chemical Soc2024

Hierarchical Protofilament Intertwining Rules the Formation of Mixed‐Curvature Amyloid Polymorphs

Giovanni Dietler, Henning Paul-Julius Stahlberg, Serguei Sekatski, Meltem Tatli, Francesco Simone Ruggeri, Jiangtao Zhou

Amyloid polymorphism is a hallmark of almost all amyloid species, yet the mechanisms underlying the formation of amyloid polymorphs and their complex architectures remain elusive. Commonly, two main mesoscopic topologies are found in amyloid polymorphs cha ...

Wiley2024

Effect of antibiotics on mechanical properties of Bordetella pertussis examined by atomic force microscopy

Giovanni Dietler, Sandor Kasas, María Inés Villalba, Leonardo Venturelli

In recent years, the coevolution of microorganisms with current antibiotics has increased the mechanisms of bacterial resistance, generating a major health problem worldwide. Bordetella pertussis is a bacterium that causes whooping cough and is capable of ...

PERGAMON-ELSEVIER SCIENCE LTD2022

Environmental Control of Amyloid Polymorphism by Modulation of Hydrodynamic Stress

Giovanni Dietler, François Gallaire, Sandor Kasas, Serguei Sekatski, Ludovic Pierre Gustave Keiser, Jiangtao Zhou, Leonardo Venturelli

The phenomenon of amyloid polymorphism is a key feature of protein aggregation. Unravelling this phenomenon is of great significance for understanding the underlying molecular mechanisms associated with neurodegenerative diseases and for the development of ...

AMER CHEMICAL SOC2021

The Nt17 Domain and its Helical Conformation Regulate the Aggregation, Cellular Properties and Neurotoxicity of Mutant Huntingtin Exon 1

Giovanni Dietler, Hilal Lashuel, Anne-Laure Mahul Mellier, Francesco Simone Ruggeri, Anass Chiki, Sean Michael Deguire, Urszula Beata Cendrowska, Sophie Vieweg, Nathan Alain Denis Riguet

Converging evidence points to the N-terminal domain comprising the first 17 amino acids of the Huntingtin protein (Nt17) as a key regulator of its aggregation, cellular properties and toxicity. In this study, we further investigated the interplay between N ...

Elsevier2021

Nanomechanical bio-sensing for fast and reliable detection of viability and susceptibility of microorganisms

Giovanni Dietler, Sandor Kasas, María Inés Villalba, Leonardo Venturelli, Raphaël Foschia

The metabolic activity of living organisms results in cellular motion on a nanometric scale that can be efficiently detected by micro- and nano- fabricated sensors. Quartz crystal microbalance and atomic force microscopy (AFM) inspired techniques have rece ...

2021

Nanomotion Spectroscopy as a New Approach to Characterize Bacterial Virulence

Giovanni Dietler, Sandor Kasas, María Inés Villalba, Giovanni Longo, Leonardo Venturelli

Atomic force microscopy (AFM)-based nanomotion detection is a label-free technique that has been used to monitor the response of microorganisms to antibiotics in a time frame of minutes. The method consists of attaching living organisms onto an AFM cantile ...

MDPI2021

Structural and DNA binding properties of mycobacterial integration host factor mIHF

Stewart Cole, David Lyndon Emsley, Giovanni Dietler, Luciano Andres Abriata, Jérémie Piton, Aleksandre Japaridze, Moreno Lelli, Nina Theres Odermatt, Rajkumar Singh

In bacteria, nucleoid associated proteins (NAPs) take part in active chromosome organization by supercoil management, three-dimensional DNA looping and direct transcriptional control. Mycobacterial integration host factor (mIHF, rv1388) is a NAP restricted ...

Elsevier2020

Effects of sedimentation, microgravity, hydrodynamic mixing and air-water interface on alpha-synuclein amyloid formation

Giovanni Dietler, Giovanni Longo, Jiangtao Zhou, Francesco Simone Ruggeri

The formation of amyloid fibrils is a characterizing feature of a range of protein misfolding diseases, including Parkinson's disease. The propensity of native proteins to form such amyloid fibril, both in vitro and in vivo, is highly sensitive to the surr ...

2020

Yeast Nanometric Scale Oscillations Highlights Fibronectin Induced Changes in C. albicans

Giovanni Dietler, Sandor Kasas, Anne-Céline Verenna Kohler, Leonardo Venturelli

Yeast resistance to antifungal drugs is a major public health issue. Fungal adhesion onto the host mucosal surface is still a partially unknown phenomenon that is modulated by several actors among which fibronectin plays an important role. Targeting the ye ...

2020