Publications de Nicolas Pavillon

A Practical Inverse-Problem Approach to Digital Holographic Reconstruction

Michaël Unser, Christian Depeursinge, Nicolas Pavillon, Emrah Bostan, Aurélien Bourquard

In this paper, we propose a new technique for high-quality reconstruction from single digital holographic acquisitions. The unknown complex object field is found as the solution of a nonlinear inverse problem that consists in the minimization of an energy ...

OSA2013

Apparatus and method for quantitative phase tomography through linear scanning with coherent and non-coherent detection

Christian Depeursinge, Nicolas Pavillon

The disclosed invention describes a new apparatus performing a new data acquisition for quantitative refractive index tomography. It is based on a linear scanning of the specimen, opposed to the classical approaches based on rotations of either the sample ...

2013

Marker-free phase nanoscopy

Pierre Magistretti, Christian Depeursinge, Pierre Marquet, Nicolas Pavillon, Pascal Jourdain, Daniel Boss, Yann Jérôme Michel Pascal Cotte, Muhammed Fatih Toy

We introduce a microscopic method that determines quantitative optical properties beyond the optical diffraction limit and allows direct imaging of unstained living biological specimens. In established holographic microscopy, complex fields are measured us ...

Nature Publishing Group2013

Single-shot, simultaneous incoherent and holographic microscopy

Christian Depeursinge, Nicolas Pavillon, Etienne Shaffer

In this work, we demonstrate single-shot, simultaneous recording and subsequent retrieval of one incoherent and two holographic (intensity and phase) images from the same camera frame. Demultiplexing of incoherent and holographic signals in the spatial fre ...

Wiley-Blackwell2012

Early Cell Death Detection with Digital Holographic Microscopy

Pierre Magistretti, Christian Depeursinge, Pierre Marquet, Nicolas Pavillon, Pascal Jourdain, Corinne Moratal

Background: Digital holography provides a non-invasive measurement of the quantitative phase shifts induced by cells in culture, which can be related to cell volume changes. It has been shown previously that regulation of cell volume, in particular as it r ...

Public Library of Science2012

Local demodulation of holograms using the Riesz transform with application to microscopy

Michaël Unser, Christian Depeursinge, Nicolas Pavillon, Chandra Sekhar Seelamantula

We propose a Riesz transform approach to the demodulation of digital holograms. The Riesz transform is a higher-dimensional extension of the Hilbert transform and is steerable to a desired orientation. Accurate demodulation of the hologram requires a relia ...

Optical Society of America2012

Exact Complex-Wave Reconstruction in Digital Holography

Michaël Unser, Christian Depeursinge, Nicolas Pavillon, Chandra Sekhar Seelamantula

We address the problem of exact complex-wave reconstruction in digital holography. We show that, by confining the object-wave modulation to one quadrant of the frequency domain, and by maintaining a reference-wave intensity higher than that of the object, ...

OSA2011

Cellular Dynamics and Three-Dimensional Refractive Index Distribution Studied with Quantitative Phase Imaging

Nicolas Pavillon

We present in this PhD thesis work various applications of digital holographic microscopy (DHM), an imaging technique based on coherent illumination which enables the recovery of the full complex wavefront, i.e. the amplitude and phase of a wave field whic ...

EPFL2011

Complex index refraction tomography with sub ?/6-resolution

Christian Depeursinge, Nicolas Pavillon, Yann Jérôme Michel Pascal Cotte

The present invention discloses a method to improve the image resolution of a microscope. This improvement is based on the mathematical processing of the complex field computed from the measurements with a microscope of the wave emitted or scattered by the ...

2011

Determination of Transmembrane Water Fluxes in Neurons Elicited by Glutamate Ionotropic Receptors and by the Co-transporters KCC2 and NKCC1: A Digital Holographic Microscopy Study

Pierre Magistretti, Christian Depeursinge, Benjamin Rappaz, Pierre Marquet, Nicolas Pavillon, Pascal Jourdain, Daniel Boss, Corinne Moratal

Digital holographic microscopy (DHM) is a noninvasive optical imaging technique that provides quantitative phase images of living cells. In a recent study, we showed that the quantitative monitoring of the phase signal by DHM was a simple label-free method ...

Society for Neuroscience2011