Publications de Thomas Gronemeyer

Mutants of o6-alkylguanine-dna alkyltransferase

Kai Johnsson, Christian Heinis, Alexandre Juillerat, Christopher Chidley, Thomas Gronemeyer, Antje Keppler

The invention relates to AGT mutants showing, when compared to the wild type human AGT, two or more advantageous properties selected from (a) reduced DNA interaction (b) localisation of the expressed protein in eukaryotic cells that is no longer restricted ...

2006

Directed evolution of O6-alkylguanine-DNA alkyltransferase for applications in protein labeling

Kai Johnsson, Christian Heinis, Alexandre Juillerat, Christopher Chidley, Thomas Gronemeyer

The specific reaction of O6-alkylguanine-DNA alkyltransferase (AGT) with O6-benzylguanine (BG) derivatives allows for a specific labeling of AGT fusion proteins with chemically diverse compounds in living cells and in vitro. The efficiency of the labeling ...

Oxford University Press2006

Degradation studies and directed evolution of the human O6-alkylguanine-DNA-alkyltransferase

Thomas Gronemeyer

A new technology for the specific and covalent labelling of fusion proteins with small organic molecules in vivo and in vitro has recently been developed in our laboratory. The method is based on the genetically encoded fusion of a target protein to O6-alk ...

EPFL2005

Adding value to fusion proteins through covalent labeling

Kai Johnsson, Thomas Gronemeyer

A review. Recombinant proteins are often expressed with an addnl. polypeptide the role of which is to aid purifn., detection, or functional studies. Recently, the role of these tags has been extended to mediate the labeling of the protein of interest with ...

2005