Publications de Martin Bertschinger

Contactless Respiration Monitoring in Real-Time via a Video Camera

Alia Lemkaddem, Martin Bertschinger, Olivier Grossenbacher

Until today, vital signs monitoring in neonatal intensive care units (NICUs) is based on wired sensors, known to cause discomfort and false alarms. In view of overcoming such issues we investigate a contactless method for respiration monitoring by means of ...

SPRINGER-VERLAG SINGAPORE PTE LTD2018

The kinetics of polyethylenimine-mediated transfection in suspension cultures of Chinese hamster ovary cells

Florian Maria Wurm, David Hacker, Martin Bertschinger, Jean Cevey

The kinetics of polyethylenimine (PEI)-mediated gene transfer at early times after transfection of Chinese hamster ovary (CHO) cell in suspension were investigated using a novel in vitro assay. Addition of an excess of competitor DNA to the culture medium ...

Humana Press Inc, Springer2008

Disassembly of polyethylenimine-DNA particles in vitro: implications for polyethylenimine-mediated DNA delivery

Florian Maria Wurm, David Hacker, Martin Jordan, Martin Bertschinger, Gaurav Backliwal

Here a simple in vitro assay was used to investigate the disassembly of nanoparticles of polyethylenimine (PEI) and DNA. Particles were formed with various PEIs, allowed to mature for 10 min, and then exposed to different competitors (RNA, DNA, BSA or hepa ...

2006

The use of RNA interference to increase PEI-mediated transient gene expression in mammalian cells

Martin Bertschinger

DNA uptake by polyethylenimine (PEI)-mediated transfection was investigated in Chinese hamster ovary (CHO DG44) cells. Rapid DNA uptake was observed with the maximum occurring during the first 45-60 min after addition of PEI-DNA complexes to cells. With lo ...

EPFL2006

Multiple glycerol shocks increase the calcium phosphate transfection of non-synchronized CHO cells

Florian Maria Wurm, David Hacker, Martin Bertschinger, Frédéric Grosjean

The exposure of CHO DG44 cells to an osmotic shock, after DNA uptake, results in a cellular volume decrease of approx. 55%. Repetitive osmotic shocks targeted different sub-populations of cells as was demonstrated using two different fluorescent reporter g ...

2006

Genetic characterization of CHO production host DG44 and derivative recombinant cell lines

Florian Maria Wurm, David Hacker, Madiha Derouazi, Martin Bertschinger

The dihydrofolate reductase-deficient Chinese hamster ovary (CHO) cell line DG44 is the dominant mammalian host for recombinant protein manufacturing, in large part because of the availability of a well-characterized genetic selection and amplification sys ...

2006

Polyethylenimine-based quality control assay for plasmid DNA

Florian Maria Wurm, David Hacker, Martin Jordan, Martin Bertschinger, Gaurav Backliwal

2006

Reduction of adenovirus E1A mRNA by RNAi results in enhanced recombinant protein expression in transiently transfected HEK293 cells

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Martin Bertschinger

Human embryonic kidney 293 (HEK293) cells, a widely used host for large-scale transient expression of recombinant proteins, are transformed with the adenovirus E1A and E1B genes. Because the E1A proteins function as transcriptional activators or repressors ...

2004