Publications de Harald Hirling

Morbillivirus Glycoprotein Expression Induces ER Stress, Alters Ca2+ Homeostasis and Results in the Release of Vasostatin

Although the pathology of Morbillivirus in the central nervous system (CNS) is well described, the molecular basis of neurodegenerative events still remains poorly understood. As a model to explore Morbillivirus-mediated CNS dysfunctions, we used canine di ...

Public Library of Science2012

Phosphorylation of synucleins by members of the Polo-like kinase family

Hilal Lashuel, Harald Hirling, Abid Oueslati, Martial Mbefo Kamdem, Magdalena Zweckstetter

Phosphorylation of alpha-synuclein (alpha-syn) at Ser-129 is a hallmark of Parkinson disease and related synucleinopathies. However, the identity of the natural kinases and phosphatases responsible for regulating alpha-syn phosphorylation remain unknown. H ...

American Society for Biochemistry and Molecular Biology2010

Calpain hydrolysis of alpha- and beta2-adaptins decreases clathrin-dependent endocytosis and may promote neurodegeneration

Harald Hirling, Nikita Rudinskiy

Clathrin-dependent endocytosis is mediated by a tightly regulated network of molecular interactions that provides essential protein-protein and protein-lipid binding activities. Here we report the hydrolysis of the alpha- and beta2-subunits of the tetramer ...

American Society for Biochemistry and Molecular Biology2009

AMPA receptor imaging by infrared scanning near-field optical microscopy

Giorgio Margaritondo, Kai Johnsson, Harald Hirling, Johanna Generosi, Michel Kropf

Infrared scanning near-field optical microscopy is used to detect AMPA-type glutamate receptor clusters in hippocampal neuron cells. AMPA's GluR2-subunits were marked with the fluorophore Alexa488 and high-resolution micrographs were taken at 6.25 µm wavel ...

Wiley2008

The somatodendritic endosomal regulator NEEP21 facilitates axonal targeting of L1/NgCAM

Harald Hirling

Correct targeting of proteins to axons and dendrites is crucial for neuronal function. We showed previously that axonal accumulation of the cell adhesion molecule L1/neuron-glia cell adhesion molecule (NgCAM) depends on endocytosis (Wisco, D., E.D. Anderso ...

Rockefeller University Press2008

Photobleaching-free infrared near-field microscopy localizes molecules in neurons

Giorgio Margaritondo, Kai Johnsson, Harald Hirling, Johanna Generosi, Michel Kropf

High-resolution detection of specific molecules in cells is a major challenge in biology. We show that infrared scanning near-field microscopy can detect the spatial distribution of alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid–type glutamate r ...

2008

Harald Hirling

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Morbillivirus Glycoprotein Expression Induces ER Stress, Alters Ca2+ Homeostasis and Results in the Release of Vasostatin

Phosphorylation of synucleins by members of the Polo-like kinase family

Calpain hydrolysis of alpha- and beta2-adaptins decreases clathrin-dependent endocytosis and may promote neurodegeneration

AMPA receptor imaging by infrared scanning near-field optical microscopy

The somatodendritic endosomal regulator NEEP21 facilitates axonal targeting of L1/NgCAM

Photobleaching-free infrared near-field microscopy localizes molecules in neurons

Subunit-specific surface mobility of differentially labeled AMPA receptor subunits

Interactions between NEEP21, GRIP1 and GluR2 regulate sorting and recycling of the glutamate receptor subunit GluR2

Superficial and deep changes of cellular mechanical properties following cytoskeleton disassembly

AMPA receptor imaging by infrared scanning near-field optical microscopy

Photobleaching-free infrared near-field microscopy localizes molecules in neurons

Morbillivirus Glycoprotein Expression Induces ER Stress, Alters Ca2+ Homeostasis and Results in the Release of Vasostatin

Phosphorylation of synucleins by members of the Polo-like kinase family

Calpain hydrolysis of alpha- and beta2-adaptins decreases clathrin-dependent endocytosis and may promote neurodegeneration

The somatodendritic endosomal regulator NEEP21 facilitates axonal targeting of L1/NgCAM

Superficial and deep changes of cellular mechanical properties following cytoskeleton disassembly

Interactions between NEEP21, GRIP1 and GluR2 regulate sorting and recycling of the glutamate receptor subunit GluR2

Subunit-specific surface mobility of differentially labeled AMPA receptor subunits